RNA aptamers to a protease, subtilisin.

RNA ligands (RNA aptamers) to a protease subtilisin were selected from pools of random RNA using SELEX (Systematic evolution of ligands by exponential enrichment). We selected RNA aptamers using a subtilisin-immobilized Sepharose column. After several rounds of selection, we obtained RNA aptamers that specifically inhibit the subtilisin activity. Characterization of the RNA aptamers will be reported.