Literature DB >> 9582429

Characterization of two Sp1 binding sites of the human sex determining SRY promoter.

M Desclozeaux1, F Poulat, P de Santa Barbara, S Soullier, P Jay, P Berta, B Boizet-Bonhoure.   

Abstract

To investigate the molecular basis of the human SRY gene regulation, we have examined the significance of two potential binding sites for the transcription factor Sp1 (Sp1A: -124 to -131 and Sp1B: -147 to -154) by DNase I footprinting and gel mobility shift assays. Cotransfection experiments in Drosophila SL2 cells implicated Sp1 protein in the transcriptional activation of the SRY promoter.

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Year:  1998        PMID: 9582429     DOI: 10.1016/s0167-4781(98)00041-4

Source DB:  PubMed          Journal:  Biochim Biophys Acta        ISSN: 0006-3002


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