Literature DB >> 9580709

New positive/negative selectable markers for mammalian cells on the basis of Blasticidin deaminase-thymidine kinase fusions.

C Karreman1.   

Abstract

Two positive and negative selectable markers were created for use in mammalian cells. They are based on two genes for the resistance to Blasticidin S (BlaS) and on the thymidine kinase (Tk) gene of herpes simplex virus (HSV). The markers can be selected positively by their ability to induce BlaS resistance and negatively on the induced sensitivity towards gancyclovir (GANC). Both constructs are also expressed in Escherichia coli and transfer BlaS resistance to this organism as well, making these markers very suitable for the construction of shuttle vectors.

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Year:  1998        PMID： 9580709      PMCID： PMC147567          DOI： 10.1093/nar/26.10.2508

Source DB:  PubMed          Journal:  Nucleic Acids Res        ISSN： 0305-1048            Impact factor:   16.971


  26 in total

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Journal:  Cell       Date:  1977-05       Impact factor: 41.582

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Authors:  M Brielmeier; J M Béchet; M H Falk; M Pawlita; A Polack; G W Bornkamm
Journal:  Nucleic Acids Res       Date:  1998-05-01       Impact factor: 16.971

3.  Cre-stimulated recombination at loxP-containing DNA sequences placed into the mammalian genome.

Authors:  B Sauer; N Henderson
Journal:  Nucleic Acids Res       Date:  1989-01-11       Impact factor: 16.971

4.  Selection against expression of the Escherichia coli gene gpt in hprt+ mouse teratocarcinoma and hybrid cells.

Authors:  C Besnard; E Monthioux; J Jami
Journal:  Mol Cell Biol       Date:  1987-11       Impact factor: 4.272

5.  Stability of HPRT marker gene expression at different gene-targeted loci: observing and overcoming a position effect.

Authors:  D W Melton; A M Ketchen; J Selfridge
Journal:  Nucleic Acids Res       Date:  1997-10-01       Impact factor: 16.971

6.  Expression of the blasticidin S deaminase gene (bsr) in tobacco: fungicide tolerance and a new selective marker for transgenic plants.

Authors:  T Kamakura; K Yoneyama; I Yamaguchi
Journal:  Mol Gen Genet       Date:  1990-09

7.  Targeting of an inducible toxic phenotype in animal cells.

Authors:  E Borrelli; R Heyman; M Hsi; R M Evans
Journal:  Proc Natl Acad Sci U S A       Date:  1988-10       Impact factor: 11.205

8.  Efficient production of Cre-mediated site-directed recombinants through the utilization of the puromycin resistance gene, pac: a transient gene-integration marker for ES cells.

Authors:  M Taniguchi; M Sanbo; S Watanabe; I Naruse; M Mishina; T Yagi
Journal:  Nucleic Acids Res       Date:  1998-01-15       Impact factor: 16.971

9.  Alterations of the hprt gene in human in vivo-derived 6-thioguanine-resistant T lymphocytes.

Authors:  R J Albertini; J P O'Neill; J A Nicklas; N H Heintz; P C Kelleher
Journal:  Nature       Date:  1985 Jul 25-31       Impact factor: 49.962

10.  Expression of human genes for adenine phosphoribosyltransferase and hypoxanthine-guanine phosphoribosyltransferase after genetic transformation of mouse cells with purified human DNA.

Authors:  S C Lester; S K LeVan; C Steglich; R DeMars
Journal:  Somatic Cell Genet       Date:  1980-03
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3.  Streptomyces lividans blasticidin S deaminase and its application in engineering a blasticidin S-producing strain for ease of genetic manipulation.

Authors:  Li Li; Jun Wu; Zixin Deng; T Mark Zabriskie; Xinyi He
Journal:  Appl Environ Microbiol       Date:  2013-02-01       Impact factor: 4.792

4.  Application of counter-selectable marker PIGA in engineering designer deletion cell lines and characterization of CRISPR deletion efficiency.

Authors:  Donghui Li; Xiaoji Sun; Fangzhou Yu; Mary Ann Perle; David Araten; Jef D Boeke
Journal:  Nucleic Acids Res       Date:  2021-03-18       Impact factor: 16.971
  4 in total

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