PURPOSE: To characterize ocular abnormalities associated with iris atrophy in DBA/2J mice and to determine whether mice of this strain develop elevated intraocular pressure (IOP) and glaucoma. METHODS: Different approaches, including slit-lamp biomicroscopy, ophthalmoscopic examination, ultrasound backscatter microscopy, and histology were used to examine the eyes of DBA/2J mice ranging from 2 to 30 months old. IOP was measured in DBA/2J mice of different ages. RESULTS: DBA/2J mice were found to develop pigment dispersion, iris transillumination, iris atrophy, anterior synechias, and elevated IOP. IOP was elevated in most mice by the age of 9 months. These changes were followed by the death of retinal ganglion cells, optic nerve atrophy, and optic nerve cupping. The prevalence and severity of these lesions increased with age. Optic nerve atrophy and optic nerve cupping was present in the majority of mice by the age of 22 months. CONCLUSIONS: DBA/2J mice develop a progressive form of secondary angle-closure glaucoma that appears to be initiated by iris atrophy and the associated formation of synechias. This mouse strain represents a useful model to evaluate mechanisms of pressure-related ganglion cell death and optic nerve atrophy, and to evaluate strategies for neuroprotection.
PURPOSE: To characterize ocular abnormalities associated with iris atrophy in DBA/2J mice and to determine whether mice of this strain develop elevated intraocular pressure (IOP) and glaucoma. METHODS: Different approaches, including slit-lamp biomicroscopy, ophthalmoscopic examination, ultrasound backscatter microscopy, and histology were used to examine the eyes of DBA/2J mice ranging from 2 to 30 months old. IOP was measured in DBA/2J mice of different ages. RESULTS: DBA/2J mice were found to develop pigment dispersion, iris transillumination, iris atrophy, anterior synechias, and elevated IOP. IOP was elevated in most mice by the age of 9 months. These changes were followed by the death of retinal ganglion cells, optic nerve atrophy, and optic nerve cupping. The prevalence and severity of these lesions increased with age. Optic nerve atrophy and optic nerve cupping was present in the majority of mice by the age of 22 months. CONCLUSIONS: DBA/2J mice develop a progressive form of secondary angle-closure glaucoma that appears to be initiated by iris atrophy and the associated formation of synechias. This mouse strain represents a useful model to evaluate mechanisms of pressure-related ganglion cell death and optic nerve atrophy, and to evaluate strategies for neuroprotection.
Authors: Mao Mao; Adam Hedberg-Buenz; Demelza Koehn; Simon W M John; Michael G Anderson Journal: Invest Ophthalmol Vis Sci Date: 2011-04-01 Impact factor: 4.799
Authors: B S Kim; O V Savinova; M V Reedy; J Martin; Y Lun; L Gan; R S Smith; S I Tomarev; S W John; R L Johnson Journal: Mol Cell Biol Date: 2001-11 Impact factor: 4.272
Authors: Wei Huang; John B Fileta; Adam Dobberfuhl; Theodoros Filippopolous; Yan Guo; Gina Kwon; Cynthia L Grosskreutz Journal: Proc Natl Acad Sci U S A Date: 2005-08-15 Impact factor: 11.205
Authors: John Danias; Fran Shen; Manolis Kavalarakis; Bin Chen; David Goldblum; Kevin Lee; Maria-Florencia Zamora; YanLing Su; Scott E Brodie; Steven M Podos; Thom Mittag Journal: Exp Eye Res Date: 2005-08-16 Impact factor: 3.467
Authors: Michael G Anderson; Richard T Libby; Douglas B Gould; Richard S Smith; Simon W M John Journal: Proc Natl Acad Sci U S A Date: 2005-03-09 Impact factor: 11.205
Authors: Benjamin J Frankfort; A Kareem Khan; Dennis Y Tse; Inyoung Chung; Ji-Jie Pang; Zhuo Yang; Ronald L Gross; Samuel M Wu Journal: Invest Ophthalmol Vis Sci Date: 2013-01-28 Impact factor: 4.799