OBJECTIVE: To evaluate the usefulness of the gamma-interferon assay in the diagnosis of caprine tuberculosis in comparison with a single intradermal tuberculin test, and to obtain a group of animals free from this infection in a herd with a high prevalence. DESIGN: An immunological study involving four serial comparative gamma-interferon and single intradermal tuberculin tests. ANIMALS: A herd of 87 goats of Guadarrama breed. PROCEDURE: Serial testing and segregation of animals. RESULTS: We found that the number of infections detected by the gamma-interferon test was considerably greater than the number detected by the single intradermal tuberculin test. A group of 10 animals was negative to both tests in two consecutive rounds and three kids were negative in the last round of testing. CONCLUSIONS: Gamma-interferon assay is appropriate for diagnosis and eradication of tuberculosis in goats. This test is able to detect early Mycobacterium bovis infection. Avian reactors with simultaneous increased reaction to bovine PPD in the gamma-interferon assay (designated as avian reactors) should be considered test positive for M bovis. By serial testing with the gamma-interferon and the single intradermal tuberculin tests, and a policy of segregation of kids at birth, it is possible to achieve a group of animals test negative for tuberculosis from a herd of goats with high immunoreactivity to this infection.
OBJECTIVE: To evaluate the usefulness of the gamma-interferon assay in the diagnosis of caprine tuberculosis in comparison with a single intradermal tuberculin test, and to obtain a group of animals free from this infection in a herd with a high prevalence. DESIGN: An immunological study involving four serial comparative gamma-interferon and single intradermal tuberculin tests. ANIMALS: A herd of 87 goats of Guadarrama breed. PROCEDURE: Serial testing and segregation of animals. RESULTS: We found that the number of infections detected by the gamma-interferon test was considerably greater than the number detected by the single intradermal tuberculin test. A group of 10 animals was negative to both tests in two consecutive rounds and three kids were negative in the last round of testing. CONCLUSIONS: Gamma-interferon assay is appropriate for diagnosis and eradication of tuberculosis in goats. This test is able to detect early Mycobacterium bovis infection. Avian reactors with simultaneous increased reaction to bovine PPD in the gamma-interferon assay (designated as avian reactors) should be considered test positive for M bovis. By serial testing with the gamma-interferon and the single intradermal tuberculin tests, and a policy of segregation of kids at birth, it is possible to achieve a group of animals test negative for tuberculosis from a herd of goats with high immunoreactivity to this infection.
Authors: Rea Tschopp; Abraham Aseffa; Esther Schelling; Stefan Berg; Elena Hailu; Endalamaw Gadisa; Meseret Habtamu; Kifle Argaw; Jakob Zinsstag Journal: PLoS One Date: 2010-08-17 Impact factor: 3.240
Authors: Joseba M Garrido; Iker A Sevilla; Beatriz Beltrán-Beck; Esmeralda Minguijón; Cristina Ballesteros; Ruth C Galindo; Mariana Boadella; Konstantin P Lyashchenko; Beatriz Romero; Maria Victoria Geijo; Francisco Ruiz-Fons; Alicia Aranaz; Ramón A Juste; Joaquín Vicente; José de la Fuente; Christian Gortázar Journal: PLoS One Date: 2011-09-14 Impact factor: 3.240
Authors: Bernat Pérez de Val; Miquel Nofrarías; Sergio López-Soria; Joseba M Garrido; H Martin Vordermeier; Bernardo Villarreal-Ramos; Maite Martín; Eugenia Puentes; Ramón A Juste; Mariano Domingo Journal: BMC Vet Res Date: 2012-10-16 Impact factor: 2.741