Positioning of nuclei in the secondary Mycelium of Schizophyllum commune in relation to differential gene expression.

In this paper we propose a novel type of gene regulation in the MATA|l4 MATB|l4 heterokaryon of Schizophyllum commune by means of differential positioning of the nuclei. It was found that binucleate hyphae with juxtaposed nuclei secrete SC4 hydrophobin (abundant during fruit-body formation), while SC3 (abundant during aerial hyphae formation in both mono- and dikaryons) appeared to be absent. Certain growth conditions disrupted the binucleate state in that the compatible nuclei became separated at a considerable distance. Under these conditions SC4 was not secreted while SC3 was secreted to a high degree. Disruption of the binucleate state was earlier observed in developing aerial hyphae which secrete SC3. Apparently, when the nuclei are in close proximity the dikaryon-expressed genes are switched on by interaction of the products of the MATA and MATB mating-type genes, while SC3 is suppressed by interacting products of the MATB genes, as occurs in the common MATA heterokaryon (MATA= MATB|l4). Growth conditions that lead to disruption of the binucleate state apparently result in abolishment of interaction between the MATB mating-type genes. Under these conditions, dikaryon-specific mRNAs do not accumulate in the MATA|l4 MATB|l4 heterokaryon, while SC3 mRNA becomes highly abundant. Copyright 1998 Academic Press.