Literature DB >> 9572978

Cloning of the ribosomal protein L41 gene of Phaffia rhodozyma and its use a drug resistance marker for transformation.

I G Kim1, S K Nam, J H Sohn, S K Rhee, G H AN, S H Lee, E S Choi.   

Abstract

The ribosomal protein L41 gene of Phaffia rhodozyma was cloned and used as a dominant selectable marker for cycloheximide resistance in the transformation of P. rhodozyma. Electrotransformation with a plasmid containing a ribosomal DNA fragment as a targeting signal typically yielded 800 to 1,200 transformants/microgram of DNA with an integrated copy number of about seven per haploid genome.

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Year:  1998        PMID: 9572978      PMCID: PMC106257          DOI: 10.1128/AEM.64.5.1947-1949.1998

Source DB:  PubMed          Journal:  Appl Environ Microbiol        ISSN: 0099-2240            Impact factor:   4.792


  12 in total

1.  Isolation of Phaffia rhodozyma Mutants with Increased Astaxanthin Content.

Authors:  G H An; D B Schuman; E A Johnson
Journal:  Appl Environ Microbiol       Date:  1989-01       Impact factor: 4.792

2.  Drastic alteration of cycloheximide sensitivity by substitution of one amino acid in the L41 ribosomal protein of yeasts.

Authors:  S Kawai; S Murao; M Mochizuki; I Shibuya; K Yano; M Takagi
Journal:  J Bacteriol       Date:  1992-01       Impact factor: 3.490

3.  High copy number integration into the ribosomal DNA of the yeast Phaffia rhodozyma.

Authors:  J Wery; D Gutker; A C Renniers; J C Verdoes; A J van Ooyen
Journal:  Gene       Date:  1997-01-03       Impact factor: 3.688

4.  Structural and phylogenetic analysis of the actin gene from the yeast Phaffia rhodozyma.

Authors:  J Wery; M J Dalderup; J Ter Linde; T Boekhout; A J Van Ooyen
Journal:  Yeast       Date:  1996-06-15       Impact factor: 3.239

5.  Molecular and genetic analysis of URA5 transformants of Cryptococcus neoformans.

Authors:  A Varma; J C Edman; K J Kwon-Chung
Journal:  Infect Immun       Date:  1992-03       Impact factor: 3.441

6.  A transformation system for the yeast Candida utilis: use of a modified endogenous ribosomal protein gene as a drug-resistant marker and ribosomal DNA as an integration target for vector DNA.

Authors:  K Kondo; T Saito; S Kajiwara; M Takagi; N Misawa
Journal:  J Bacteriol       Date:  1995-12       Impact factor: 3.490

7.  The major promoter element of rRNA transcription in yeast lies 2 kb upstream.

Authors:  E A Elion; J R Warner
Journal:  Cell       Date:  1984-12       Impact factor: 41.582

8.  Two different genes from Schwanniomyces occidentalis determine ribosomal resistance to cycloheximide.

Authors:  L Del Pozo; D Abarca; J Hoenicka; A Lmenez
Journal:  Eur J Biochem       Date:  1993-04-15

9.  Natural cycloheximide resistance in yeast. The role of ribosomal protein L41.

Authors:  P Dehoux; J Davies; M Cannon
Journal:  Eur J Biochem       Date:  1993-04-15

10.  Perfect state of Rhodomyces dendrorhous (Phaffia rhodozyma).

Authors:  W I Golubev
Journal:  Yeast       Date:  1995-02       Impact factor: 3.239

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  8 in total

1.  Metallopeptidase Stp1 activates the transcription factor Sre1 in the carotenogenic yeast Xanthophyllomyces dendrorhous.

Authors:  Melissa Gómez; María Soledad Gutiérrez; Ana María González; Carla Gárate-Castro; Dionisia Sepúlveda; Salvador Barahona; Marcelo Baeza; Víctor Cifuentes; Jennifer Alcaíno
Journal:  J Lipid Res       Date:  2019-12-05       Impact factor: 5.922

2.  Integrative transformation system for the metabolic engineering of the sphingoid base-producing yeast Pichia ciferrii.

Authors:  Jung-Hoon Bae; Jung-Hoon Sohn; Chang-Seo Park; Joon-Shick Rhee; Eui-Sung Choi
Journal:  Appl Environ Microbiol       Date:  2003-02       Impact factor: 4.792

3.  Identification and functional characterization of the CYP51 gene from the yeast Xanthophyllomyces dendrorhous that is involved in ergosterol biosynthesis.

Authors:  Kritsye Leiva; Nicole Werner; Dionisia Sepúlveda; Salvador Barahona; Marcelo Baeza; Víctor Cifuentes; Jennifer Alcaíno
Journal:  BMC Microbiol       Date:  2015-04-25       Impact factor: 3.605

4.  Genome editing enables reverse genetics of multicellular development in the choanoflagellate Salpingoeca rosetta.

Authors:  David S Booth; Nicole King
Journal:  Elife       Date:  2020-06-04       Impact factor: 8.140

5.  Damage response protein 1 (Dap1) functions in the synthesis of carotenoids and sterols in Xanthophyllomyces dendrorhous.

Authors:  Ana-María González; Maximiliano Venegas; Salvador Barahona; Melissa Gómez; María-Soledad Gutiérrez; Dionisia Sepúlveda; Marcelo Baeza; Víctor Cifuentes; Jennifer Alcaíno
Journal:  J Lipid Res       Date:  2022-02-02       Impact factor: 5.922

6.  Enhancement of carotenoid production by disrupting the C22-sterol desaturase gene (CYP61) in Xanthophyllomyces dendrorhous.

Authors:  Iris Loto; María Soledad Gutiérrez; Salvador Barahona; Dionisia Sepúlveda; Pilar Martínez-Moya; Marcelo Baeza; Víctor Cifuentes; Jennifer Alcaíno
Journal:  BMC Microbiol       Date:  2012-10-18       Impact factor: 3.605

7.  Cloning of the cytochrome p450 reductase (crtR) gene and its involvement in the astaxanthin biosynthesis of Xanthophyllomyces dendrorhous.

Authors:  Jennifer Alcaíno; Salvador Barahona; Marisela Carmona; Carla Lozano; Andrés Marcoleta; Mauricio Niklitschek; Dionisia Sepúlveda; Marcelo Baeza; Víctor Cifuentes
Journal:  BMC Microbiol       Date:  2008-10-06       Impact factor: 3.605

8.  Increase in the astaxanthin synthase gene (crtS) dose by in vivo DNA fragment assembly in Xanthophyllomyces dendrorhous.

Authors:  Gabriela Contreras; Salvador Barahona; María Cecilia Rojas; Marcelo Baeza; Víctor Cifuentes; Jennifer Alcaíno
Journal:  BMC Biotechnol       Date:  2013-10-09       Impact factor: 2.563

  8 in total

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