BACKGROUND: One common feature of renal diseases is the development of interstitial fibrosis, but the mechanism of this process remains undefined. We hypothesized that platelet-activating factor (PAF), a classical acute inflammatory mediator involved in the pathogenesis of renal damage, acts on renal tubulointerstitial cells, contributing to the development of fibrosis. For this reason we evaluated the effect of PAF on matrix regulation and cell-growth-related events in tubulointerstitial cells. METHODS: In vitro studies were conducted with two tubulointerstitial cell lines: renal tubuloepithelial cells (NRK 52E) and interstitial fibroblasts (NRK 49F). The effect of PAF on extracellular matrix gene expression was determined by Northern blot. Fibronectin synthesis was quantified by metabolic labelling and immunoprecipitation. Cell growth changes were evaluated by fluorescence-activated cell-sorting analysis (cell cycle and size) and total protein content by 3[H]leucine incorporation. RESULTS: In renal tubuloepithelial cells and interstitial fibroblasts, PAF increased fibronectin mRNA expression. PAF-effect on the expression of collagen genes differed depending on the cell type studied. In tubuloepithelial cells there was an increase in type I and IV collagen mRNA levels, while only type I collagen was increased in fibroblasts. The overexpression of matrix proteins induced by PAF was completely blocked by preincubation of cells with the PAF receptor antagonist, BN52021. The PAF-induced upregulation of fibronectin expression was correlated with the increase in fibronectin synthesis. These effects were not associated with an increase in hyperplasia (characterized by changes in cell cycle) either in tubuloepithelial cells or in interstitial fibroblasts. Moreover, PAF did not induce tubular hypertrophy (changes in protein content and cell size). CONCLUSIONS: Our data suggest that PAF could be a mediator involved in extracellular matrix accumulation and, therefore, participate in the formation of renal interstitial fibrosis.
BACKGROUND: One common feature of renal diseases is the development of interstitial fibrosis, but the mechanism of this process remains undefined. We hypothesized that platelet-activating factor (PAF), a classical acute inflammatory mediator involved in the pathogenesis of renal damage, acts on renal tubulointerstitial cells, contributing to the development of fibrosis. For this reason we evaluated the effect of PAF on matrix regulation and cell-growth-related events in tubulointerstitial cells. METHODS: In vitro studies were conducted with two tubulointerstitial cell lines: renal tubuloepithelial cells (NRK 52E) and interstitial fibroblasts (NRK 49F). The effect of PAF on extracellular matrix gene expression was determined by Northern blot. Fibronectin synthesis was quantified by metabolic labelling and immunoprecipitation. Cell growth changes were evaluated by fluorescence-activated cell-sorting analysis (cell cycle and size) and total protein content by 3[H]leucine incorporation. RESULTS: In renal tubuloepithelial cells and interstitial fibroblasts, PAF increased fibronectin mRNA expression. PAF-effect on the expression of collagen genes differed depending on the cell type studied. In tubuloepithelial cells there was an increase in type I and IV collagen mRNA levels, while only type I collagen was increased in fibroblasts. The overexpression of matrix proteins induced by PAF was completely blocked by preincubation of cells with the PAF receptor antagonist, BN52021. The PAF-induced upregulation of fibronectin expression was correlated with the increase in fibronectin synthesis. These effects were not associated with an increase in hyperplasia (characterized by changes in cell cycle) either in tubuloepithelial cells or in interstitial fibroblasts. Moreover, PAF did not induce tubular hypertrophy (changes in protein content and cell size). CONCLUSIONS: Our data suggest that PAF could be a mediator involved in extracellular matrix accumulation and, therefore, participate in the formation of renal interstitial fibrosis.
Authors: Matheus Correa-Costa; Vinicius Andrade-Oliveira; Tarcio T Braga; Angela Castoldi; Cristhiane F Aguiar; Clarice S T Origassa; Andrea C D Rodas; Meire I Hiyane; Denise M A C Malheiros; Francisco J O Rios; Sonia Jancar; Niels O S Câmara Journal: Lab Invest Date: 2014-02-03 Impact factor: 5.662
Authors: Jun Mo Kang; Hyun Seob Lee; Junghyun Kim; Dong Ho Yang; Hye Yun Jeong; Yu Ho Lee; Dong Jin Kim; Seon Hwa Park; MinJi Sung; Jaehee Kim; Hyun Ju An; Sang Ho Lee; So Young Lee Journal: J Korean Med Sci Date: 2020-09-14 Impact factor: 2.153