T Jeren1, A Vince. 1. Dr. Fran Mihaljević University Hospital for Infectious Diseases, University of Zagreb, Croatia.
Abstract
OBJECTIVE: To evaluate the dynamics of cytologic changes in the cerebrospinal fluid (CSF) of patients with tick-borne encephalitis (TBE) and to determine the various forms of mononuclear cells by immunocytochemical methods. STUDY DESIGN: To perform a cytologic analysis of 200 cells in stained sediment of CSF. To determine the population and subpopulation of mononuclear cells by using monoclonal antibodies. RESULTS: Pleocytosis in CSF in patients with TBE during the first three days of illness was, on average, 570 cells per cubic millimeter with about 60% neutrophils. Mononuclear cells predominated from the fifth day of illness. Among lymphoid cells, 18% carried marker CD20 and 60% of them marker CD3. Among monocytoid cells, 66% carried marker CD32. CONCLUSION: CSF cytology findings reflect the immunologic and inflammatory changes in the CNS during TBE.
OBJECTIVE: To evaluate the dynamics of cytologic changes in the cerebrospinal fluid (CSF) of patients with tick-borne encephalitis (TBE) and to determine the various forms of mononuclear cells by immunocytochemical methods. STUDY DESIGN: To perform a cytologic analysis of 200 cells in stained sediment of CSF. To determine the population and subpopulation of mononuclear cells by using monoclonal antibodies. RESULTS: Pleocytosis in CSF in patients with TBE during the first three days of illness was, on average, 570 cells per cubic millimeter with about 60% neutrophils. Mononuclear cells predominated from the fifth day of illness. Among lymphoid cells, 18% carried marker CD20 and 60% of them marker CD3. Among monocytoid cells, 66% carried marker CD32. CONCLUSION: CSF cytology findings reflect the immunologic and inflammatory changes in the CNS during TBE.