[The detection of 10-acyl derivatives of phenothiazine by staining reactions and a spectrophotometric method for their hydrolysis products].

Identification of phenothiazine 10-acyl derivatives by staining and spectrophotometry of their hydrolysis products 2-chlorophenothiazine, 2-trifluoromethylphenothiazine, and 2-carboxyaminophenothiazine appreciably improved the sensitivity and specificity of their detection. Staining on silufol plates detects at least 0.05 to 0.15 microgram per spot. The specific parameters of hydrolysis products absorption are notably superior to those of the native compounds and are (in chloroform, U1%1 cm): 1821 for 2-chlorophenothiazine, 1400 for 2-trifluoromethylphenothiazine, and 1656 for 2-carboxyaminophenothiazine.