Literature DB >> 9566214

Separation and mapping of chromosomes of parasitic protozoa.

R Hernandez-Rivas1, A Scherf.   

Abstract

Many protozoan parasites represent an important group of human pathogens. Pulsed Field Gradient Gel Electrophoresis (PFGE) analysis has been an important tool for fundamental genetic studies of parasites like Trypanosoma, Leishmania, Giardia or the human malaria parasite Plasmodium falciparum. We present PFGE conditions allowing a high resolution separation of chromosomes ranging from 500 to 4000 kb within a two day electrophoresis run. In addition, we present conditions for separating large chromosomes (2000-6000 kb) within 36 hr. We demonstrate that the application of two dimensional PFGE (2D-PFGE) technique to parasite karyotypes is a very useful method for the analysis of dispersed gene families and comparative studies of the intrachromosomal genome organization.

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Year:  1997        PMID： 9566214     DOI： 10.1590/s0074-02761997000600017

Source DB:  PubMed          Journal:  Mem Inst Oswaldo Cruz        ISSN： 0074-0276            Impact factor:   2.743


  8 in total

1.  In-gel DNA radiolabelling and two-dimensional pulsed field gel electrophoresis procedures suitable for fingerprinting and mapping small eukaryotic genomes.

Authors:  J F Brugère; E Cornillot; G Méténier; C P Vivarès
Journal:  Nucleic Acids Res       Date:  2000-05-15       Impact factor: 16.971

2.  A central role for Plasmodium falciparum subtelomeric regions in spatial positioning and telomere length regulation.

Authors:  Luisa M Figueiredo; Lúcio H Freitas-Junior; Emmanuel Bottius; Jean-Christophe Olivo-Marin; Artur Scherf
Journal:  EMBO J       Date:  2002-02-15       Impact factor: 11.598

3.  Encephalitozoon cuniculi (Microspora) genome: physical map and evidence for telomere-associated rDNA units on all chromosomes.

Authors:  J F Brugère; E Cornillot; G Méténier; A Bensimon; C P Vivarès
Journal:  Nucleic Acids Res       Date:  2000-05-15       Impact factor: 16.971

4.  Antigenic variation in malaria: in situ switching, relaxed and mutually exclusive transcription of var genes during intra-erythrocytic development in Plasmodium falciparum.

Authors:  A Scherf; R Hernandez-Rivas; P Buffet; E Bottius; C Benatar; B Pouvelle; J Gysin; M Lanzer
Journal:  EMBO J       Date:  1998-09-15       Impact factor: 11.598

5.  A single member of the Plasmodium falciparum var multigene family determines cytoadhesion to the placental receptor chondroitin sulphate A.

Authors:  Nicola K Viebig; Benoit Gamain; Christine Scheidig; Catherine Lépolard; Jude Przyborski; Michael Lanzer; Jürg Gysin; Artur Scherf
Journal:  EMBO Rep       Date:  2005-08       Impact factor: 8.807

6.  DNA secondary structures are associated with recombination in major Plasmodium falciparum variable surface antigen gene families.

Authors:  Adam F Sander; Thomas Lavstsen; Thomas S Rask; Michael Lisby; Ali Salanti; Sarah L Fordyce; Jakob S Jespersen; Richard Carter; Kirk W Deitsch; Thor G Theander; Anders Gorm Pedersen; David E Arnot
Journal:  Nucleic Acids Res       Date:  2013-11-18       Impact factor: 16.971

7.  Functional analysis of sirtuin genes in multiple Plasmodium falciparum strains.

Authors:  Catherine J Merrick; Rays H Y Jiang; Kristen M Skillman; Upeka Samarakoon; Rachel M Moore; Ron Dzikowski; Michael T Ferdig; Manoj T Duraisingh
Journal:  PLoS One       Date:  2015-03-17       Impact factor: 3.240

8.  Mitotic evolution of Plasmodium falciparum shows a stable core genome but recombination in antigen families.

Authors:  Selina E R Bopp; Micah J Manary; A Taylor Bright; Geoffrey L Johnston; Neekesh V Dharia; Fabio L Luna; Susan McCormack; David Plouffe; Case W McNamara; John R Walker; David A Fidock; Eros Lazzerini Denchi; Elizabeth A Winzeler
Journal:  PLoS Genet       Date:  2013-02-07       Impact factor: 5.917
  8 in total

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