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Literature DB >> 956383

Purification and properties of sulfite oxidase from human liver.

Abstract

Sulfite oxidase has been purified to near homogeneity from human liver. Properties of the molecule have been investigated and compared to those of the rat liver enzyme which has been isolated in a pure form. Both proteins exist as dimeric molecules with one molybdenum and one cytochrome b5-type heme per sub-unit. The human enzyme has a slightly larger subunit molecular weight (61,100 vs. 57,200 daltons) and is a more negatively charged molecule. Decreased reactivity of the human enzyme with various electron acceptors suggests the presence of nonfunctional molybdenum centers in a portion of the molecules isolated. Human liver sulfite oxidase cross-reacts strongly with antibody prepared against the rat liver enzyme. Human enzyme activity is precipitated by antibody at concentrations about twofold greater than required for comparable complexation of rat sulfite oxidase.

Entities: Chemical Gene Species

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Year: 1976 PMID： 956383 PMCID： PMC333211 DOI： 10.1172/JCI108499

Source DB: PubMed Journal: J Clin Invest ISSN： 0021-9738 Impact factor: 14.808

20 in total

1. AN APPARATUS FOR PREPARATIVE TEMPERATURE-REGULATED POLYACRYLAMIDE GEL ELECTROPHORESIS.

Authors: T JOVIN; A CHRAMBACH; M A NAUGHTON
Journal: Anal Biochem Date: 1964-11 Impact factor: 3.365

2. DISC ELECTROPHORESIS. II. METHOD AND APPLICATION TO HUMAN SERUM PROTEINS.

Authors: B J DAVIS
Journal: Ann N Y Acad Sci Date: 1964-12-28 Impact factor: 5.691

3. The nonhemoglobin erythrocytic proteins, studied by electrophoresis on starch gel.

Authors: A HAUT; G R TUDHOPE; G E CARTWRIGHT
Journal: J Clin Invest Date: 1962-03 Impact factor: 14.808

4. Protein measurement with the Folin phenol reagent.

Authors: O H LOWRY; N J ROSEBROUGH; A L FARR; R J RANDALL
Journal: J Biol Chem Date: 1951-11 Impact factor: 5.157

5. Molecular basis of the biological function of molybdenum. Molybdenum-free sulfite oxidase from livers of tungsten-treated rats.

Authors: J L Johnson; H J Cohen; K V Rajagopalan
Journal: J Biol Chem Date: 1974-08-25 Impact factor: 5.157

6. Molecular basis of the biological function of molybdenum. Effect of tungsten on xanthine oxidase and sulfite oxidase in the rat.

Authors: J L Johnson; K V Rajagopalan; H J Cohen
Journal: J Biol Chem Date: 1974-02-10 Impact factor: 5.157

7. Molecular basis of the biological function of molybdenum. Developmental patterns of sulfite oxidase and xanthine oxidase in the rat.

Authors: H J Cohen; J L Johnson; K V Rajagopalan
Journal: Arch Biochem Biophys Date: 1974-10 Impact factor: 4.013

8. Hepatic sulfite oxidase. Effect of anions on interaction with cytochrome c.

Authors: D L Kessler; K V Rajagopalan
Journal: Biochim Biophys Acta Date: 1974-12-29

9. The use of diaminobenzidine for spectrophotometric and acrylamide gel detection of sulfite oxidase and its applicability to hydrogen peroxide-generating enzymes.

Authors: H J Cohen
Journal: Anal Biochem Date: 1973-05 Impact factor: 3.365

10. Sulfite oxidase deficiency in man: demonstration of the enzymatic defect.

Authors: S H Mudd; F Irreverre; L Laster
Journal: Science Date: 1967-06-23 Impact factor: 47.728

14 in total

1. Combined deficiency of xanthine oxidase and sulphite oxidase: a defect of molybdenum metabolism or transport?

Authors: M Duran; F A Beemer; C van de Heiden; J Korteland; P K de Bree; M Brink; S K Wadman; I Lombeck
Journal: J Inherit Metab Dis Date: 1978 Impact factor: 4.982

2. Urinary thiosulphate/creatinine concentration ratio in hospitalized children.

Authors: A P Reynolds; R A Harkness
Journal: J Inherit Metab Dis Date: 1991 Impact factor: 4.982

3. Attempt at treatment with tetrahydrobiopterin in combined deficiency of xanthine oxidase and sulphite oxidase.

Authors: M Fujitaka; N Sakura; K Ueda; H Konishi; S Yoshida; T Yamasaki
Journal: J Inherit Metab Dis Date: 1991 Impact factor: 4.982

4. Structure-based alteration of substrate specificity and catalytic activity of sulfite oxidase from sulfite oxidation to nitrate reduction.

Authors: James A Qiu; Heather L Wilson; K V Rajagopalan
Journal: Biochemistry Date: 2012-01-30 Impact factor: 3.162

5. Combined deficiency of xanthine oxidase and sulphite oxidase: diagnosis of a new case followed by an antenatal diagnosis.

Authors: P Desjacques; B Mousson; C Vianey-Liaud; R Boulieu; C Bory; P Baltassat; P Divry; M T Zabot; J Cotte; P Lagier
Journal: J Inherit Metab Dis Date: 1985 Impact factor: 4.982

6. Molybdopterin in carbon monoxide oxidase from carboxydotrophic bacteria.

Authors: O Meyer; K V Rajagopalan
Journal: J Bacteriol Date: 1984-02 Impact factor: 3.490

7. Distribution of selected essential (Co, Cu, Fe, Mn, Mo, Se, and Zn) and nonessential (Cd, Pb) trace elements among protein fractions from hepatic cytosol of European chub (Squalius cephalus L.).

Authors: Nesrete Krasnići; Zrinka Dragun; Marijana Erk; Biserka Raspor
Journal: Environ Sci Pollut Res Int Date: 2012-08-11 Impact factor: 4.223