Literature DB >> 956355

Competition by 24,25-dihydroxycholecalciferol in the competitive protein binding radioassay of 25-hydroxycalciferol.

J G Haddad, C Min, J Walgate, T Hahn.   

Abstract

24,25-dihydroxycholecalciferol (24,25-(OH)2D3) is equipotent to 25-hydroxycholecalciferol in the displacement of 3H25-OHD3 from rat serum binding sites, and is extracted by the ethanol procedure recommended in non-chromatographic competitive protein binding radioassays for 25-OHD. Serum 24,25-(OH)2D content was measured following 24,25-(OH)2D3 isolation from lipid extracts by liquid-gel partition chromatography. Since normal serum 24,25-(OH)2D concentration is appreciable, non-chromatographic 25-OHD assays most probably overestimate serum 25-OHD levels as a result of their recognition of 24,25-(OH)2D3.

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Year:  1976        PMID： 956355     DOI： 10.1210/jcem-43-3-712

Source DB:  PubMed          Journal:  J Clin Endocrinol Metab        ISSN： 0021-972X            Impact factor:   5.958


  7 in total

1.  Serum 25-hydroxy vitamin D in normal and osteomalacic subjects: a comparison of two assay techniques.

Authors:  B Murray; R Freaney
Journal:  Ir J Med Sci       Date:  1979-01       Impact factor: 1.568

2.  Clinical utility of simultaneous quantitation of 25-hydroxyvitamin D and 24,25-dihydroxyvitamin D by LC-MS/MS involving derivatization with DMEQ-TAD.

Authors:  Martin Kaufmann; J Christopher Gallagher; Munro Peacock; Karl-Peter Schlingmann; Martin Konrad; Hector F DeLuca; Rita Sigueiro; Borja Lopez; Antonio Mourino; Miguel Maestro; René St-Arnaud; Joel S Finkelstein; Donald P Cooper; Glenville Jones
Journal:  J Clin Endocrinol Metab       Date:  2014-03-26       Impact factor: 5.958

3.  [Influence of vitamin D therapy on renal osteodystrophy in children (author's transl)].

Authors:  M Bulla; G J Stock; G Delling; H Hofmann; G Offermann
Journal:  Klin Wochenschr       Date:  1980-03-03

4.  Regulation of the metabolism of 25-hydroxyvitamin D3 in primary cultures of chick kidney cells.

Authors:  U Trechsel; J P Bonjour; H Fleisch
Journal:  J Clin Invest       Date:  1979-07       Impact factor: 14.808

5.  Determination of vitamin D and its metabolites in plasma from normal and anephric man.

Authors:  R M Shepard; R L Horst; A J Hamstra; H F DeLuca
Journal:  Biochem J       Date:  1979-07-15       Impact factor: 3.857

6.  Inhibition of parathyroid hormone secretion by 25-hydroxycholecalciferol and 24,25-dihydroxycholecalciferol in the dog.

Authors:  J M Canterbury; S Lerman; A J Claflin; H Henry; A Norman; E Reiss
Journal:  J Clin Invest       Date:  1978-05       Impact factor: 14.808

7.  25-Hydroxycholecalciferol serum levels in breast-fed infants.

Authors:  J A Birkbeck; H F Scott
Journal:  Arch Dis Child       Date:  1980-09       Impact factor: 3.791
  7 in total

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