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Literature DB >> 9559861

Estimation of membrane potential deltapsi in reconstituted plasma membrane vesicles using a numerical model of oxonol VI distribution.

Abstract

A model of membrane potential-dependent distribution of oxonol VI to estimate the electrical potential difference deltapsi across Schizosaccharomyces pombe plasma membrane vesicles (PMV) has been developed. deltapsi was generated by the H+-ATPase reconstituted in the PMV. The model treatment was necessary since the usual calibration of the dye fluorescence changes by diffusion potentials (K+ + valinomycin) failed. The model allows for fitting of fluorescence changes at different vesicle and dye concentrations, yielding deltapsi in ATP-energized PMV of 80 mV. The described model treatment to estimate deltapsi may be applicable for other reconstituted membrane systems.

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Year: 1997 PMID： 9559861 DOI： 10.1023/a:1022439203461

Source DB: PubMed Journal: J Bioenerg Biomembr ISSN： 0145-479X Impact factor: 2.945

14 in total

1. Ion-dependent generation of the electrochemical proton gradient delta muH+ in reconstituted plasma membrane vesicles from the yeast Metschnikowia reukaufii.

Authors: H U Gläser; M Höfer
Journal: Biochim Biophys Acta Date: 1987-12-11

Review 2. Reconstitution of ion-motive transport ATPases in artificial lipid membranes.

Authors: A Villalobo
Journal: Biochim Biophys Acta Date: 1990-05-15

3. Two mechanisms by which fluorescent oxonols indicate membrane potential in human red blood cells.

Authors: P R Pratap; T S Novak; J C Freedman
Journal: Biophys J Date: 1990-04 Impact factor: 4.033

4. Transport mechanism of hydrophobic ions through lipid bilayer membranes.

Authors: B Ketterer; B Neumcke; P Läuger
Journal: J Membr Biol Date: 1971-09 Impact factor: 1.843

Review 5. Potential-sensitive molecular probes in membranes of bioenergetic relevance.

Authors: J C Smith
Journal: Biochim Biophys Acta Date: 1990-03-15

Review 6. Slow fluorescent indicators of membrane potential: a survey of different approaches to probe response analysis.

Authors: J Plásek; K Sigler
Journal: J Photochem Photobiol B Date: 1996-04 Impact factor: 6.252

7. Oxonol VI as an optical indicator for membrane potentials in lipid vesicles.

Authors: H J Apell; B Bersch
Journal: Biochim Biophys Acta Date: 1987-10-16

8. Use of oxonol V as a probe of membrane potential in proteoliposomes containing cytochrome oxidase in the submitochondrial orientation.

Authors: C E Cooper; D Bruce; P Nicholls
Journal: Biochemistry Date: 1990-04-24 Impact factor: 3.162

Estimation of membrane potential deltapsi in reconstituted plasma membrane vesicles using a numerical model of oxonol VI distribution.

1. Ion-dependent generation of the electrochemical proton gradient delta muH+ in reconstituted plasma membrane vesicles from the yeast Metschnikowia reukaufii.

Review 2. Reconstitution of ion-motive transport ATPases in artificial lipid membranes.

3. Two mechanisms by which fluorescent oxonols indicate membrane potential in human red blood cells.

4. Transport mechanism of hydrophobic ions through lipid bilayer membranes.

Review 5. Potential-sensitive molecular probes in membranes of bioenergetic relevance.

Review 6. Slow fluorescent indicators of membrane potential: a survey of different approaches to probe response analysis.

7. Oxonol VI as an optical indicator for membrane potentials in lipid vesicles.

8. Use of oxonol V as a probe of membrane potential in proteoliposomes containing cytochrome oxidase in the submitochondrial orientation.

9. The mechanism of voltage-sensitive dye responses on sarcoplasmic reticulum.

10. Oxonol-V as a probe of chromaffin granule membrane potentials.

1. Computational models for monitoring the trans-membrane potential with fluorescent probes: the DiSC₃(5) case.

Review 2. Fluorescence techniques for determination of the membrane potentials in high throughput screening.