Literature DB >> 9557875

Purification and properties of recombinant beta-galactosidase from Bacillus circulans.

H Fujimoto1, M Miyasato, Y Ito, T Sasaki, K Ajisaka.   

Abstract

A gene encoding beta-galactosidase from Bacillus circulans which had hydrolysis specificity for the beta1-3 linkage was expressed in Escherichia coli. The beta-galactosidase was purified from crude cell lysates of E. coli by column chromatographies on Resource Q and Sephacryl S-200 HR. The enzyme released galactose with high selectivity from oligosaccharides which had terminal beta1-3 linked galactose residues. However it did not hydrolyse beta1-4 linked galactooligosaccharides. Moreover, Galbeta1-3GlcNAc, Galbeta1-3GalNAc, and their p-nitrophenyl glycosides were regioselectively synthesized in 10-46% yield by the transglycosylation reaction using this enzyme.

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Year:  1998        PMID： 9557875     DOI： 10.1023/a:1006916222187

Source DB:  PubMed          Journal:  Glycoconj J        ISSN： 0282-0080            Impact factor:   2.916


  30 in total

1.  Cell surface beta-1,4-galactosyltransferase is associated with the detergent-insoluble cytoskeleton on migrating mesenchymal cells.

Authors:  D J Eckstein; B D Shur
Journal:  Exp Cell Res       Date:  1992-07       Impact factor: 3.905

2.  Genomic structure and expression of human beta-1,4-galactosyltransferase.

Authors:  L Mengle-Gaw; M F McCoy-Haman; D C Tiemeier
Journal:  Biochem Biophys Res Commun       Date:  1991-05-15       Impact factor: 3.575

3.  Isolation of mononuclear cells and granulocytes from human blood. Isolation of monuclear cells by one centrifugation, and of granulocytes by combining centrifugation and sedimentation at 1 g.

Authors:  A Böyum
Journal:  Scand J Clin Lab Invest Suppl       Date:  1968

4.  Cleavage of structural proteins during the assembly of the head of bacteriophage T4.

Authors:  U K Laemmli
Journal:  Nature       Date:  1970-08-15       Impact factor: 49.962

5.  Hybridization of denatured RNA and small DNA fragments transferred to nitrocellulose.

Authors:  P S Thomas
Journal:  Proc Natl Acad Sci U S A       Date:  1980-09       Impact factor: 11.205

6.  Characterization of a family of structurally related glycoproteins expressing beta 1-6-branched asparagine-linked oligosaccharides in human colon carcinoma cells.

Authors:  S Laferté; L C Loh
Journal:  Biochem J       Date:  1992-04-01       Impact factor: 3.857

7.  A functional study of purified CD4+ and CD8+ cells isolated from synovial fluid of patients with rheumatoid arthritis and other arthritides.

Authors:  J Hovdenes; G Gaudernack; T K Kvien; T Egeland; O J Mellbye
Journal:  Scand J Immunol       Date:  1989-06       Impact factor: 3.487

8.  Expression of beta-1,4-galactosyltransferase gene during 3T3 cell growth.

Authors:  A S Masibay; G P Damewood; E Boeggeman; P K Qasba
Journal:  Biochim Biophys Acta       Date:  1991-10-08

9.  Characterization of two cis-regulatory regions in the murine beta 1,4-galactosyltransferase gene. Evidence for a negative regulatory element that controls initiation at the proximal site.

Authors:  A Harduin-Lepers; J H Shaper; N L Shaper
Journal:  J Biol Chem       Date:  1993-07-05       Impact factor: 5.157

10.  Beta 1-6 branching of Asn-linked oligosaccharides is directly associated with metastasis.

Authors:  J W Dennis; S Laferté; C Waghorne; M L Breitman; R S Kerbel
Journal:  Science       Date:  1987-05-01       Impact factor: 47.728
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  5 in total

1.  Facile enzymatic conversion of lactose into lacto-N-tetraose and lacto-N-neotetraose.

Authors:  T Murata; T Inukai; M Suzuki; M Yamagishi; A T Usui
Journal:  Glycoconj J       Date:  1999-03       Impact factor: 2.916

2.  A novel cold-adapted β-galactosidase isolated from Halomonas sp. S62: gene cloning, purification and enzymatic characterization.

Authors:  Guo-Xiang Wang; Yun Gao; Bo Hu; Xiao-Ling Lu; Xiao-Yu Liu; Bing-Hua Jiao
Journal:  World J Microbiol Biotechnol       Date:  2013-03-14       Impact factor: 3.312

3.  Linkage of sugar chains to a fragment peptide of FGF-5S by a chemoenzymatic strategy and changes in the rate of proteolytic hydrolysis.

Authors:  K Ajisaka; M Miyasato; C Ito; Y Fujita; Y Yamazaki; S Oka
Journal:  Glycoconj J       Date:  2001-04       Impact factor: 2.916

4.  Unique Glycoform-Dependent Monoclonal Antibodies for Mouse Mucin 21.

Authors:  Jun Nishida; Shigeyuki Shichino; Tatsuya Tsukui; Mayumi Hoshino; Tomoko Okada; Kyoko Okada; Yuri Yi; Seiko Toraya-Brown; Miho Mochizuki; Ryouta Koizumi; Katrin Ishii-Schrade; Kaori Denda-Nagai; Tatsuro Irimura
Journal:  Int J Mol Sci       Date:  2022-06-16       Impact factor: 6.208

5.  Characterization of the Streptococcus pneumoniae BgaC protein as a novel surface beta-galactosidase with specific hydrolysis activity for the Galbeta1-3GlcNAc moiety of oligosaccharides.

Authors:  Jae Kap Jeong; Ohsuk Kwon; Yun Mi Lee; Doo-Byoung Oh; Jung Mi Lee; Seonghun Kim; Eun-Hye Kim; Tu Nhat Le; Dong-Kwon Rhee; Hyun Ah Kang
Journal:  J Bacteriol       Date:  2009-03-06       Impact factor: 3.490
  5 in total

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