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Literature DB >> 9557738

Role of the N-terminal zinc finger of human immunodeficiency virus type 1 nucleocapsid protein in virus structure and replication.

V Tanchou¹, D Decimo, C Péchoux, D Lener, V Rogemond, L Berthoux, M Ottmann, J L Darlix.

Abstract

Nucleocapsid protein (NCp7) of human immunodeficiency virus type 1 is found covering the genomic RNA in the interior of the viral particle. It is a highly basic protein with two zinc fingers of the form CX2CX4HX4C which exhibit strong affinity for a zinc cation. To study the structure-function relationship of the N-terminal zinc finger of NCp7, this domain was either deleted or changed to CX2CX4CX4C. We examined virus formation and structure as well as proviral DNA synthesis. Our data show that these two NC mutations result in the formation of particles with an abnormal core morphology and impair the end of proviral DNA synthesis, leading to noninfectious viruses.

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Year: 1998 PMID： 9557738 PMCID： PMC109678 DOI： 10.1128/JVI.72.5.4442-4447.1998

Source DB: PubMed Journal: J Virol ISSN： 0022-538X Impact factor: 5.103

50 in total

1. Recombinant HIV-1 nucleocapsid protein accelerates HIV-1 reverse transcriptase catalyzed DNA strand transfer reactions and modulates RNase H activity.

Authors: J A Peliska; S Balasubramanian; D P Giedroc; S J Benkovic
Journal: Biochemistry Date: 1994-11-22 Impact factor: 3.162

2. Trans-activation of the 5' to 3' viral DNA strand transfer by nucleocapsid protein during reverse transcription of HIV1 RNA.

Authors: J L Darlix; A Vincent; C Gabus; H de Rocquigny; B Roques
Journal: C R Acad Sci III Date: 1993-08

3. Mutations in the N-terminal domain of human immunodeficiency virus type 1 nucleocapsid protein affect virion core structure and proviral DNA synthesis.

Authors: L Berthoux; C Péchoux; M Ottmann; G Morel; J L Darlix
Journal: J Virol Date: 1997-09 Impact factor: 5.103

4. Characterization of human immunodeficiency virus type 1 dimeric RNA from wild-type and protease-defective virions.

Authors: W Fu; R J Gorelick; A Rein
Journal: J Virol Date: 1994-08 Impact factor: 5.103

5. DNA strand exchange and selective DNA annealing promoted by the human immunodeficiency virus type 1 nucleocapsid protein.

Authors: Z Tsuchihashi; P O Brown
Journal: J Virol Date: 1994-09 Impact factor: 5.103

6. Fusion from without directed by human immunodeficiency virus particles.

Authors: F Clavel; P Charneau
Journal: J Virol Date: 1994-02 Impact factor: 5.103

7. 1H NMR structure and biological studies of the His23-->Cys mutant nucleocapsid protein of HIV-1 indicate that the conformation of the first zinc finger is critical for virus infectivity.

Authors: H Déméné; C Z Dong; M Ottmann; M C Rouyez; N Jullian; N Morellet; Y Mely; J L Darlix; M C Fournié-Zaluski; S Saragosti
Journal: Biochemistry Date: 1994-10-04 Impact factor: 3.162

8. Mapping of functionally important residues of a cysteine-histidine box in the human immunodeficiency virus type 1 nucleocapsid protein.

Authors: T Dorfman; J Luban; S P Goff; W A Haseltine; H G Göttlinger
Journal: J Virol Date: 1993-10 Impact factor: 5.103

9. Transactivation of the minus-strand DNA transfer by nucleocapsid protein during reverse transcription of the retroviral genome.

Authors: B Allain; M Lapadat-Tapolsky; C Berlioz; J L Darlix
Journal: EMBO J Date: 1994-02-15 Impact factor: 11.598

10. Specific binding of HIV-1 nucleocapsid protein to PSI RNA in vitro requires N-terminal zinc finger and flanking basic amino acid residues.

Authors: J Dannull; A Surovoy; G Jung; K Moelling
Journal: EMBO J Date: 1994-04-01 Impact factor: 11.598

54 in total

1. Nucleic acid chaperone activity of the ORF1 protein from the mouse LINE-1 retrotransposon.

Authors: S L Martin; F D Bushman
Journal: Mol Cell Biol Date: 2001-01 Impact factor: 4.272

2. Subtle alterations of the native zinc finger structures have dramatic effects on the nucleic acid chaperone activity of human immunodeficiency virus type 1 nucleocapsid protein.

Authors: Jianhui Guo; Tiyun Wu; Bradley F Kane; Donald G Johnson; Louis E Henderson; Robert J Gorelick; Judith G Levin
Journal: J Virol Date: 2002-05 Impact factor: 5.103

Role of the N-terminal zinc finger of human immunodeficiency virus type 1 nucleocapsid protein in virus structure and replication.

1. Recombinant HIV-1 nucleocapsid protein accelerates HIV-1 reverse transcriptase catalyzed DNA strand transfer reactions and modulates RNase H activity.

2. Trans-activation of the 5' to 3' viral DNA strand transfer by nucleocapsid protein during reverse transcription of HIV1 RNA.

3. Mutations in the N-terminal domain of human immunodeficiency virus type 1 nucleocapsid protein affect virion core structure and proviral DNA synthesis.

4. Characterization of human immunodeficiency virus type 1 dimeric RNA from wild-type and protease-defective virions.

5. DNA strand exchange and selective DNA annealing promoted by the human immunodeficiency virus type 1 nucleocapsid protein.

6. Fusion from without directed by human immunodeficiency virus particles.

7. 1H NMR structure and biological studies of the His23-->Cys mutant nucleocapsid protein of HIV-1 indicate that the conformation of the first zinc finger is critical for virus infectivity.

8. Mapping of functionally important residues of a cysteine-histidine box in the human immunodeficiency virus type 1 nucleocapsid protein.

9. Transactivation of the minus-strand DNA transfer by nucleocapsid protein during reverse transcription of the retroviral genome.

10. Specific binding of HIV-1 nucleocapsid protein to PSI RNA in vitro requires N-terminal zinc finger and flanking basic amino acid residues.

1. Nucleic acid chaperone activity of the ORF1 protein from the mouse LINE-1 retrotransposon.

2. Subtle alterations of the native zinc finger structures have dramatic effects on the nucleic acid chaperone activity of human immunodeficiency virus type 1 nucleocapsid protein.

3. Element-specific localization of Drosophila retrotransposon Gag proteins occurs in both nucleus and cytoplasm.

4. Mechanism for nucleic acid chaperone activity of HIV-1 nucleocapsid protein revealed by single molecule stretching.

5. Intracellular targeting of Gag proteins of the Drosophila telomeric retrotransposons.

6. DNA condensation by the nucleocapsid protein of HIV-1: a mechanism ensuring DNA protection.

7. Zinc finger-dependent HIV-1 nucleocapsid protein-TAR RNA interactions.

8. G-quartets direct assembly of HIV-1 nucleocapsid protein along single-stranded DNA.

Review 9. Properties and functions of the nucleocapsid protein in virus assembly.

10. Multiple effects of an anti-human immunodeficiency virus nucleocapsid inhibitor on virus morphology and replication.