Early patterning of the rat cerebral wall for regional organization of a neuronal population expressing latexin.

The exact timing of regional patterning in the developing cerebral cortex and other telencephalic structures remains to be elucidated. In the present study, we addressed this issue by comparing the distribution and density of neuronal population expressing latexin in the adult rat telencephalon, with the regional pattern in the fetal cerebral wall as to the potential to generate latexin-expressing neurons. Immunohistochemical analyses on adult animals have shown that latexin-expressing neurons are restricted to a lateral cortical field, within which they are most abundant at the middle level, decreasing in number rostrally and caudally. Substantial numbers of latexin-immunopositive neurons were recorded in the claustrum and endopiriform nuclei, both of which are located from rostral to middle level in the lateral telencephalon. By examining the number and density of latexin-immunopositive neurons in organotypic slice cultures from various portions of the developing rat cerebral wall, it has been shown that the regional pattern within the early cerebral wall as to the potential to generate latexin-expressing neurons matches well the distribution and density of latexin-expressing neurons in the adult telencephalon. Thus, in cultures derived from either embryonic day 13 or 16 fetuses, latexin-immunopositive neurons appeared most prominently in those from rostral-to-middle portions of the lateral cerebral wall, decreasing in number rostrally and caudally. In cultures from the dorsal cerebral wall, the number was generally very low. In light of our previous finding that most prospective latexin-expressing neurons are still dividing at embryonic day 13, it can be concluded that some kind of pattern formation event occurs within the early cerebral wall even prior to the genesis of the postmitotic neurons that would be later allocated in a region-specific manner.