The role of cyclin E in the regulation of entry into S phase.

Cyclin E is a crucial regulator of entry into S phase in higher eukaryotes and acts in association with the protein kinase cdk2. Cyclin E expression is transcriptionally controlled in mammalian cells resulting in a maximum just before entry into S phase. Premature expression of cyclin E advances entry into S phase, while lack of cyclin E prevents entry into S phase. Cyclin E/cdk2 activity is regulated at multiple levels (by transcription, phosphorylation and inhibitor proteins) and appears to be involved in triggering initiation of DNA replication and in regulating genes important for proliferation and progression through S phase.