| Literature DB >> 9551818 |
M A Campanero1, B Sadaba, E García-Quetglas, J R Azanza.
Abstract
A rapid, sensitive, specific liquid chromatographic method has been developed for the determination of therapeutic levels of ganciclovir in human plasma. Plasma (1 ml) and acyclovir (I.S.) were treated with 50% trichloroacetic acid. The supernatant was neutralized with 2 M NaOH and purified with chloroform. The aqueous phase (80 microl) was analyzed by a 3-microm Hypersil ODS C18 column with 0.04 M triethylamine-0.1 M sodium dihydrogen phosphate monohydrate as the mobile phase (1 ml/min) and ultraviolet detection at 254 nm. Calibration was linear from 50 to 10000 ng/ml. Intra- and inter-day C.V. did no exceed 6.65%. The detection limit was about 10 ng/ml.Entities:
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Year: 1998 PMID： 9551818 DOI： 10.1016/s0378-4347(97)00666-x
Source DB: PubMed Journal: J Chromatogr B Biomed Sci Appl ISSN： 1387-2273