Literature DB >> 955103

A simple method for the perfusion of isolated liver cells.

R Van Der Meer, J M Tager.   

Abstract

The perfusion technique described in this paper offers a new and simple method for studying liver cell metabolism in a more dynamic way. It has potential use in hormonal response studies, pulsechase experiments and many other types of investigations. Finally, the perfusion technique may be applicable to other isolated cell preparations as well. The method combines some advantages of the flow-through technique with the great advantages of using isolated liver cells, apparently without disturbing the viability of the cells.

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Year:  1976        PMID： 955103     DOI： 10.1016/0014-5793(76)80865-4

Source DB:  PubMed          Journal:  FEBS Lett        ISSN： 0014-5793            Impact factor:   4.124


  9 in total

1.  Alpha-adrenergic stimulation of glutamine metabolism in isolated rat hepatocytes.

Authors:  A J Verhoeven; J M Estrela; A J Meijer
Journal:  Biochem J       Date:  1985-09-01       Impact factor: 3.857

2.  Uncoupling effect of polyunsaturated fatty acid deficiency in isolated rat hepatocytes:effect on glycerol metabolism.

Authors:  M A Piquet; E Fontaine; B Sibille; C Filippi; C Keriel; X M Leverve
Journal:  Biochem J       Date:  1996-08-01       Impact factor: 3.857

3.  Inhibition of glycerol metabolism in hepatocytes isolated from endotoxic rats.

Authors:  P Leclercq; C Filippi; B Sibille; S Hamant; C Keriel; X M Leverve
Journal:  Biochem J       Date:  1997-07-15       Impact factor: 3.857

4.  The mitochondrial consequences of uncoupling intact cells depend on the nature of the exogenous substrate.

Authors:  B Sibille; C Filippi; M A Piquet; P Leclercq; E Fontaine; X Ronot; M Rigoulet; X Leverve
Journal:  Biochem J       Date:  2001-04-01       Impact factor: 3.857

5.  Inhibition of gluconeogenesis in isolated rat hepatocytes after chronic treatment with phenobarbital.

Authors:  D Argaud; S Halimi; F Catelloni; X M Leverve
Journal:  Biochem J       Date:  1991-12-15       Impact factor: 3.857

6.  Stimulation by glucose of gluconeogenesis in hepatocytes isolated from starved rats.

Authors:  M Rigoulet; X M Leverve; P J Plomp; A J Meijer
Journal:  Biochem J       Date:  1987-08-01       Impact factor: 3.857

7.  Oxidative phosphorylation in intact hepatocytes: quantitative characterization of the mechanisms of change in efficiency and cellular consequences.

Authors:  X Leverve; B Sibille; A Devin; M A Piquet; P Espié; M Rigoulet
Journal:  Mol Cell Biochem       Date:  1998-07       Impact factor: 3.396

8.  Interrelations between ureogenesis and gluconeogenesis in isolated hepatocytes. The role of antion transport and the competition for energy.

Authors:  A B Wojtczak; E I Wałajtys-Rode; M J Geelen
Journal:  Biochem J       Date:  1978-02-15       Impact factor: 3.857

9.  An adherent cell perifusion technique to study the overall and sequential response of rat alveolar macrophages to toxic substances.

Authors:  G Forget; M J Lacroix; A Cadieux; R Calvert; J H Grose; P Sirois
Journal:  Environ Health Perspect       Date:  1983-09       Impact factor: 9.031
  9 in total

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