Literature DB >> 955027

Large scale cultivation of a free-living nematode (Caenorhabditis elegans).

J R Vanfleteren.   

Abstract

A method is presented for the large scale cultivation of the free-living nematode Caenorhabditis elegans, using continuous aeration and agitation in glass ware (stirrer flasks) developed for the continuous culture of suspended cells. With this technique, populations up to 10(9) nematodes may be obtained in a 10 1 culture in less than 6 weeks with an inoculum of some 50 worms. Costs can be reduced by using an inexpensive yeast extract, available from the food industry.

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Year:  1976        PMID： 955027     DOI： 10.1007/BF01933985

Source DB:  PubMed          Journal:  Experientia        ISSN： 0014-4754


  7 in total

1.  Biochemical aspects of the nutrition of Caenorhabditis briggsae.

Authors:  F W SAYRE; E L HANSEN; E A YARWOOD
Journal:  Exp Parasitol       Date:  1963-04       Impact factor: 2.011

2.  Stimulatory effects of peptides on growth of the free-living nematode Caenorhabditis briggsae.

Authors:  C Pinnock; B Shane; E L Stokstad
Journal:  Proc Soc Exp Biol Med       Date:  1975-03

3.  Mass culture of axenic nematods uising continuous aeration.

Authors:  E J Buecher; E L Hansen
Journal:  J Nematol       Date:  1971-04       Impact factor: 1.402

4.  Practical methods for the axenic culture of the free-living nematodes Turbatrix aceti and Caenorhabditis briggsae.

Authors:  M Rothstein
Journal:  Comp Biochem Physiol B       Date:  1974-12-15

5.  Nematode growth factor.

Authors:  J R Vanfleteren
Journal:  Nature       Date:  1974-03-15       Impact factor: 49.962

6.  Assay of a proteinaceous growth factor required for maturation of the free-living nematode, Caenorhabditis briggsae.

Authors:  W R Lower; E Hansen; E A Yarwood
Journal:  J Exp Zool       Date:  1966-02

7.  The genetics of Caenorhabditis elegans.

Authors:  S Brenner
Journal:  Genetics       Date:  1974-05       Impact factor: 4.562
  7 in total
  5 in total

1.  Selective inhibition of reproduction in aminopterin-treated nematodes.

Authors:  J R Vanfleteren; H Avau
Journal:  Experientia       Date:  1977-07-15

2.  Sterol metabolism in the nematodeCaenorhabditis elegans.

Authors:  D J Chitwood; W R Lusby; R Lozano; M J Thompson; J A Svoboda
Journal:  Lipids       Date:  1984-07       Impact factor: 1.880

3.  Life-Span Extension by Axenic Dietary Restriction Is Independent of the Mitochondrial Unfolded Protein Response and Mitohormesis in Caenorhabditis elegans.

Authors:  Huaihan Cai; Madina Rasulova; Lieselot Vandemeulebroucke; Lea Meagher; Caroline Vlaeminck; Ineke Dhondt; Bart P Braeckman
Journal:  J Gerontol A Biol Sci Med Sci       Date:  2017-10-01       Impact factor: 6.053

4.  CBP-1 Acts in GABAergic Neurons to Double Life Span in Axenically Cultured Caenorhabditis elegans.

Authors:  Huaihan Cai; Ineke Dhondt; Lieselot Vandemeulebroucke; Caroline Vlaeminck; Madina Rasulova; Bart P Braeckman
Journal:  J Gerontol A Biol Sci Med Sci       Date:  2019-07-12       Impact factor: 6.053

5.  Axenic Culture of Caenorhabditis elegans Alters Lysosomal/Proteasomal Balance and Increases Neuropeptide Expression.

Authors:  Huaihan Cai; Ping Wu; Lieselot Vandemeulebroucke; Ineke Dhondt; Madina Rasulova; Andy Vierstraete; Bart P Braeckman
Journal:  Int J Mol Sci       Date:  2022-09-29       Impact factor: 6.208
  5 in total

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