Literature DB >> 9546218

Hydrolysis of a slow cyclic thiophosphate substrate of RNase T1 analyzed by time-resolved crystallography.

I Zegers1, R Loris, G Dehollander, A Fattah Haikal, F Poortmans, J Steyaert, L Wyns.   

Abstract

Here we present a time-resolved crystallographic analysis of the hydrolysis of exo (Sp) guanosine 2',3'-cyclophosphorothioate by RNase T1. The use of a slow substrate and fast crystallization methods made it possible to perform the study with conventional data-collection techniques. The results support the idea that the hydrolysis reaction proceeds through a mechanism that is the inverse of the transesterification reaction. In addition, the structures provide an explanation for the differential behavior of RNase T1 towards exo- and endo-cyclic thiophosphates.

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Year:  1998        PMID： 9546218     DOI： 10.1038/nsb0498-280

Source DB:  PubMed          Journal:  Nat Struct Biol        ISSN： 1072-8368


  8 in total

1.  Trimeric domain-swapped barnase.

Authors:  I Zegers; J Deswarte; L Wyns
Journal:  Proc Natl Acad Sci U S A       Date:  1999-02-02       Impact factor: 11.205

2.  Substrate specificity and mutational analysis of Kluyveromyces lactis gamma-toxin, a eukaryal tRNA anticodon nuclease.

Authors:  Ruchi Jain; Michael G Poulos; Julien Gros; Anupam K Chakravarty; Stewart Shuman
Journal:  RNA       Date:  2011-05-24       Impact factor: 4.942

3.  RNA Cleavage Properties of Nucleobase-Specific RNase MC1 and Cusativin Are Determined by the Dinucleotide-Binding Interactions in the Enzyme-Active Site.

Authors:  Priti Thakur; Jowad Atway; Patrick A Limbach; Balasubrahmanyam Addepalli
Journal:  Int J Mol Sci       Date:  2022-06-24       Impact factor: 6.208

4.  Structure-activity relationships in Kluyveromyces lactis gamma-toxin, a eukaryal tRNA anticodon nuclease.

Authors:  Niroshika Keppetipola; Ruchi Jain; Birthe Meineke; Melinda Diver; Stewart Shuman
Journal:  RNA       Date:  2009-04-21       Impact factor: 4.942

5.  Nonspecific base recognition mediated by water bridges and hydrophobic stacking in ribonuclease I from Escherichia coli.

Authors:  Sergio Martinez Rodriguez; Santosh Panjikar; Karolien Van Belle; Lode Wyns; Joris Messens; Remy Loris
Journal:  Protein Sci       Date:  2008-02-27       Impact factor: 6.725

6.  A phosphate-binding histidine of binuclear metallophosphodiesterase enzymes is a determinant of 2',3'-cyclic nucleotide phosphodiesterase activity.

Authors:  Niroshika Keppetipola; Stewart Shuman
Journal:  J Biol Chem       Date:  2008-08-28       Impact factor: 5.157

7.  Determinants of eukaryal cell killing by the bacterial ribotoxin PrrC.

Authors:  Birthe Meineke; Beate Schwer; Raffael Schaffrath; Stewart Shuman
Journal:  Nucleic Acids Res       Date:  2010-09-19       Impact factor: 16.971

Review 8.  Not making the cut: Techniques to prevent RNA cleavage in structural studies of RNase-RNA complexes.

Authors:  Seth P Jones; Christian Goossen; Sean D Lewis; Annie M Delaney; Michael L Gleghorn
Journal:  J Struct Biol X       Date:  2022-03-11
  8 in total

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