| Literature DB >> 9546216 |
M Bachelin1, G Hessler, G Kurz, J G Hacia, P B Dervan, H Kessler.
Abstract
In this work, we present the first NMR solution structure of a DNA/RNA hybrid containing stereoregular Rp-phosphorothioate modifications of all DNA backbone linkages. The complex of the enzymatically synthesized phosphorothioate DNA octamer (all-Rp)-d(GCGTCAGG) and its complementary RNA r(CCUGACGC) was found to adopt an overall conformation within the A-form family. Most helical parameters and the sugar puckers of the DNA strand assume values intermediate between A- and B-form. The close structural similarity with the unmodified DNA/RNA hybrid of the same sequence may explain why both the natural and the sulfur-substituted complex can be recognized and digested by ribonuclease H.Entities:
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Year: 1998 PMID： 9546216 DOI： 10.1038/nsb0498-271
Source DB: PubMed Journal: Nat Struct Biol ISSN： 1072-8368