Literature DB >> 9546194

Sonication-dependent electroporation of the erythromycin-producing bacterium Saccharopolyspora erythraea.

N B Fitzgerald1, R S English, J S Lampel, T J Vanden Boom.   

Abstract

We report the development of an electrotransformation method applicable to all strains of Saccharopolyspora erythraea examined to date. Vegetatively grown mycelia were rendered electrocompetent by subjecting mycelial suspensions to ultrasound pulses. The protocol provides an alternative route for the introduction of DNA into filamentous microorganisms otherwise recalcitrant to transformation techniques.

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Year:  1998        PMID： 9546194      PMCID： PMC106195          DOI： 10.1128/AEM.64.4.1580-1583.1998

Source DB:  PubMed          Journal:  Appl Environ Microbiol        ISSN： 0099-2240            Impact factor:   4.792


  9 in total

1.  Organization of the enzymatic domains in the multifunctional polyketide synthase involved in erythromycin formation in Saccharopolyspora erythraea.

Authors:  S Donadio; L Katz
Journal:  Gene       Date:  1992-02-01       Impact factor: 3.688

2.  Modular organization of genes required for complex polyketide biosynthesis.

Authors:  S Donadio; M J Staver; J B McAlpine; S J Swanson; L Katz
Journal:  Science       Date:  1991-05-03       Impact factor: 47.728

3.  Properties of in vitro recombinant derivatives of pJV1, a multi-copy plasmid from Streptomyces phaeochromogenes.

Authors:  C R Bailey; C J Bruton; M J Butler; K F Chater; J E Harris; D A Hopwood
Journal:  J Gen Microbiol       Date:  1986-08

4.  Procaryotic and eucaryotic traits of DNA methylation in spiroplasmas (mycoplasmas).

Authors:  I Nur; M Szyf; A Razin; G Glaser; S Rottem; S Razin
Journal:  J Bacteriol       Date:  1985-10       Impact factor: 3.490

Review 5.  Metabolic engineering of polyketide biosynthesis.

Authors:  C L Hershberger
Journal:  Curr Opin Biotechnol       Date:  1996-10       Impact factor: 9.740

6.  Transformation of Streptomyces erythraeus.

Authors:  H Yamamoto; K H Maurer; C R Hutchinson
Journal:  J Antibiot (Tokyo)       Date:  1986-09       Impact factor: 2.649

7.  A Simple and Rapid Method of Transformation of Streptomyces rimosus R6 and Other Streptomycetes by Electroporation.

Authors:  J Pigac; H Schrempf
Journal:  Appl Environ Microbiol       Date:  1995-01       Impact factor: 4.792

Review 8.  Polyketide synthesis: prospects for hybrid antibiotics.

Authors:  L Katz; S Donadio
Journal:  Annu Rev Microbiol       Date:  1993       Impact factor: 15.500

9.  Cloning of genes governing the deoxysugar portion of the erythromycin biosynthesis pathway in Saccharopolyspora erythraea (Streptomyces erythreus).

Authors:  J Vara; M Lewandowska-Skarbek; Y G Wang; S Donadio; C R Hutchinson
Journal:  J Bacteriol       Date:  1989-11       Impact factor: 3.490
  9 in total
  4 in total

1.  Ethyl-substituted erythromycin derivatives produced by directed metabolic engineering.

Authors:  D L Stassi; S J Kakavas; K A Reynolds; G Gunawardana; S Swanson; D Zeidner; M Jackson; H Liu; A Buko; L Katz
Journal:  Proc Natl Acad Sci U S A       Date:  1998-06-23       Impact factor: 11.205

2.  Electroporation of germinated conidia and young mycelium as an efficient transformation system for Acremonium chrysogenum.

Authors:  Jessica Cruz-Ramón; Francisco J Fernández; Armando Mejía; Francisco Fierro
Journal:  Folia Microbiol (Praha)       Date:  2018-06-25       Impact factor: 2.099

3.  Quantification of N-acetylcysteamine activated methylmalonate incorporation into polyketide biosynthesis.

Authors:  Stephan Klopries; Uschi Sundermann; Frank Schulz
Journal:  Beilstein J Org Chem       Date:  2013-04-05       Impact factor: 2.883

4.  Dam and Dcm methylations prevent gene transfer into Clostridium pasteurianum NRRL B-598: development of methods for electrotransformation, conjugation, and sonoporation.

Authors:  Jan Kolek; Karel Sedlar; Ivo Provaznik; Petra Patakova
Journal:  Biotechnol Biofuels       Date:  2016-01-20       Impact factor: 6.040
  4 in total

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