BACKGROUND: Alcohol-induced changes in haemoglobin have been suggested as potential biochemical markers of alcohol abuse. In this study, we investigated the diagnostic value of alcohol-induced haemoglobin adducts among women. METHODS: Whole (Hb fractions) and affinity-purified (AHb fractions) haemolysates from 87 women in three groups (a) social drinkers (n=31), (b) heavy drinkers (n=27) and (c) alcoholic subjects (n=29) - were analysed by HPLC-CEC. RESULTS: Three fractions (HbA 1a2, HbA1dl and AHbA1d1) showed significant differences (P<0.05) between the groups and a significant positive correlation (P<0.05) with self-reported alcohol consumption (r=0.58-0.76) as determined by the Malmö modified Michigan Alcoholism Screening Test (MmMAST) and structured CAGE questionnaire (r=0.58-0.76). HbA1a2, HbA1d1 and AHbA1d1 had specificities of 97%, 97% and 100% respectively and detected 41%, 33% and 78% of heavy drinkers with overall accuracies (OAs) of 71%, 67% and 90%. Sensitivities in the detection of alcoholic subjects were 86% (OA=92%), 76% (OA=87%) and 81 % (OA=91%) respectively. The fractions had higher OAs than traditional markers of alcohol abuse. CONCLUSION: This study indicates that at least three alcohol-induced haemoglobin adducts occurring in vivo can be measured with promising diagnostic efficiency among women.
BACKGROUND:Alcohol-induced changes in haemoglobin have been suggested as potential biochemical markers of alcohol abuse. In this study, we investigated the diagnostic value of alcohol-induced haemoglobin adducts among women. METHODS: Whole (Hb fractions) and affinity-purified (AHb fractions) haemolysates from 87 women in three groups (a) social drinkers (n=31), (b) heavy drinkers (n=27) and (c) alcoholic subjects (n=29) - were analysed by HPLC-CEC. RESULTS: Three fractions (HbA 1a2, HbA1dl and AHbA1d1) showed significant differences (P<0.05) between the groups and a significant positive correlation (P<0.05) with self-reported alcohol consumption (r=0.58-0.76) as determined by the Malmö modified Michigan Alcoholism Screening Test (MmMAST) and structured CAGE questionnaire (r=0.58-0.76). HbA1a2, HbA1d1 and AHbA1d1 had specificities of 97%, 97% and 100% respectively and detected 41%, 33% and 78% of heavy drinkers with overall accuracies (OAs) of 71%, 67% and 90%. Sensitivities in the detection of alcoholic subjects were 86% (OA=92%), 76% (OA=87%) and 81 % (OA=91%) respectively. The fractions had higher OAs than traditional markers of alcohol abuse. CONCLUSION: This study indicates that at least three alcohol-induced haemoglobin adducts occurring in vivo can be measured with promising diagnostic efficiency among women.
Authors: Juana Pinar-Sanchez; Pablo Bermejo López; Julián Solís García Del Pozo; Jose Redondo-Ruiz; Laura Navarro Casado; Fernando Andres-Pretel; María Luisa Celorrio Bustillo; Mercedes Esparcia Moreno; Santiago García Ruiz; Jose Javier Solera Santos; Beatriz Navarro Bravo Journal: J Clin Med Date: 2022-04-06 Impact factor: 4.241