Literature DB >> 9537040

A new quantitative fluorimetric assay for phagocytosis of bacteria.

B Vray1, J Hoebeke, M Saint-Guillain, R Leloup, A D Strosberg.   

Abstract

We describe a new quantitative fluorimetric assay for phagocytosis of bacteria. A suspension of fluorescein-labelled bacteria (Micrococcus lysodeikticus) is mixed and incubated with phagocytes. After fixation with paraformaldehyde, the excess non-phagocytosed and adsorbed bacteria are lysed with a solution of lysozyme in phosphate-buffered saline. After washing of the phagocytes, the fluorescence of those that have ingested the labelled bacteria is measured with a spectrofluorimeter. We report results obtained with different types of phagocytes which show that this method allows sensitivity, saturation and kinetic studies and the calculation of a phagocytic index.

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Year:  1980        PMID： 9537040     DOI： 10.1111/j.1365-3083.1980.tb00220.x

Source DB:  PubMed          Journal:  Scand J Immunol        ISSN： 0300-9475            Impact factor:   3.487


  9 in total

1.  Targeted immobilisation of lysozyme in the enamel pellicle from different solutions.

Authors:  Christian Hannig; Bettina Spitzmüller; Wiebke Hoth-Hannig; Matthias Hannig
Journal:  Clin Oral Investig       Date:  2009-12-05       Impact factor: 3.573

2.  Does diet influence salivary enzyme activities in elephant species?

Authors:  Carolin Boehlke; Sandra Pötschke; Verena Behringer; Christian Hannig; Oliver Zierau
Journal:  J Comp Physiol B       Date:  2016-08-31       Impact factor: 2.200

3.  Salivary enzyme activity in anorexic persons—a controlled clinical trial.

Authors:  Elżbieta Paszynska; Nadine Schlueter; Agnieszka Slopien; Monika Dmitrzak-Weglarz; Marta Dyszkiewicz-Konwinska; Christian Hannig
Journal:  Clin Oral Investig       Date:  2015-03-11       Impact factor: 3.573

4.  Mab. 198: a monoclonal antibody recognizing the complement type 3 receptor (CR3) in the rabbit.

Authors:  E G Smet; W De Smet; L Brys; P C De Baetselier
Journal:  Immunology       Date:  1986-11       Impact factor: 7.397

5.  Opsonic, cytotoxic, precipitating, blocking of bacterial adherence, and other activities of monoclonal IgE antibody compared with IgA and IgM.

Authors:  H Tlaskalová-Hogenová; J Simecková; V Vĕtvicka; L Fornůsek; M TalaFantová; P Mancal
Journal:  Immunology       Date:  1984-11       Impact factor: 7.397

6.  Preparation, characterization, and immunogenicity of conjugate vaccines directed against Actinobacillus pleuropneumoniae virulence determinants.

Authors:  W Byrd; S Kadis
Journal:  Infect Immun       Date:  1992-08       Impact factor: 3.441

7.  Characterisation of lysozyme activity in the in situ pellicle using a fluorimetric assay.

Authors:  Christian Hannig; Bettina Spitzmüller; Matthias Hannig
Journal:  Clin Oral Investig       Date:  2008-09-23       Impact factor: 3.573

8.  Production of NOS2 and inflammatory cytokines is reduced by selected protein kinase inhibitors with partial repolarization of HL-60 derived and human blood macrophages.

Authors:  Gábor Bögel; József Murányi; Bálint Szokol; Zoltán Kukor; István Móra; Tamás Kardon; László Őrfi; András Hrabák
Journal:  Heliyon       Date:  2021-12-27

9.  Nutritional influences on enzyme activities in saliva of Asian and African elephants.

Authors:  Carolin Boehlke; Sabrina Schuster; Lucas Kauthe; Oliver Zierau; Christian Hannig
Journal:  J Comp Physiol B       Date:  2021-07-07       Impact factor: 2.200
  9 in total

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