| Literature DB >> 9535157 |
C A Malcolm1, D Bourguet, A Ascolillo, S J Rooker, C F Garvey, L M Hall, N Pasteur, M Raymond.
Abstract
An acetylcholinesterase (AChE) gene, Ace.x, showing 93% identity of deduced amino acid sequence to Anopheles stephensi Ace has been cloned from a Culex pipiens strain homozygous for insensitive AChE (iAChE) mediated insecticide resistance. DNA sequence of genomic DNA clones identified exons 2-5. RFLP of six clones indicated four possible alleles. Linkage analysis located Ace.x to chromosome I, less than 0.8 centimorgans from the sex locus, whereas the locus conferring resistance was 2.0 centimorgans from plum-eye on chromosome II. Ace.1 coding for AChE1, which is associated with resistance, is therefore autosomal. We propose that Ace.x is the recently postulated Ace.2 coding for the biochemically distinct AChE2, which is not associated with resistance.Entities:
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Year: 1998 PMID： 9535157 DOI： 10.1046/j.1365-2583.1998.72055.x
Source DB: PubMed Journal: Insect Mol Biol ISSN： 0962-1075 Impact factor: 3.585