| Literature DB >> 953032 |
L Holmberg, B Bladh, B Astedt.
Abstract
Commercially available urokinase (EC 3.4.99.26), though highly active, is still contaminated with unrelated proteins and degradation fragments of urokinase. Further purification of a urokinase preparation by chromatography on benzamidine-Sepharose is described. The final preparation consisted of two molecular forms of urokinase with molecular weights of respectively 31 000 and 54 000. The 54 000-dalton urokinase appears to be composed of two protein chains, one of which is the 31 000-dalton urokinase. A monospecific antiserum against urokinase was raised.Entities:
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Year: 1976 PMID： 953032 DOI： 10.1016/0005-2744(76)90174-1
Source DB: PubMed Journal: Biochim Biophys Acta ISSN： 0006-3002