Effects of the thyroid state on cholesterol metabolism in the rat.

An isotopic equilibrium method which permits the in vivo measurements of cholesterol turnover processes was applied to different groups of rats: (1) radiothyroidectomized, (2) low-iodine fed, and (3) L-thyroxin fed. Plasma cholesterol concentrations were enhanced after thyroidectomy and reduced by large dose of L-thyroxin. A low-iodine diet decreased plasma thyroxin level but did not affect plasma cholesterol. Thyroid levels in plasma modified the coefficient of intestinal absorption of cholesterol. After thyroidectomy or under conditions of reduced thyroxin formation this absorption coefficient was enhanced. The absorption coefficient of cholesterol was decreased in rats receiving 31 or 61.5 mug/day of L-thyroxin but was not changed in rats fed 110 mug/day L-thyroxin. The proportion of de novo biosynthetisized cholesterol eliminated into the feces (external secretion) was reduced while thyroxin levels were low. The fecal excretion of cholesterol and the in vitro exchange of cholesterol between erythrocytes and plasma were increased by L-thyroxin ingestion. The rate of cholesterol biosynthesis was decreased after thyroidectomy and enhanced by L-thyroxin feeding. In fact, changes in thyroid state modified indirectly the biosynthesis of cholesterol by its effects on metabolism and on the coefficient of intestinal absorption of cholesterol.