| Literature DB >> 952944 |
O Kajander, T Eloranta, A Raina.
Abstract
A rapid and sensitive isotopic method is presented for the assay of S-adenosylhomocysteine hydrolase (EC 3.3.1.1) activity, based on the formation of radioactive S-adenosylhomocysteine labelled in the adenosine portion. The radioactive product is separated either by low-voltage paper electrophoresis or by using phosphocellulose ion-exchange paper. Some kinetic properties of the enzyme from rat liver have shown to be clearly different from those reported earlier for this enzyme. The use of erythro-9-(2-hydroxy-3-nonyl)adenine, a potent inhibitor of adenosine deaminase, makes it possible to measure the S-adenosylhomocysteine hydrolase activity in tissues with a high adenosine deaminase activity, e.g. in intestinal mucosa.Entities:
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Year: 1976 PMID： 952944 DOI： 10.1016/0005-2744(76)90268-0
Source DB: PubMed Journal: Biochim Biophys Acta ISSN： 0006-3002