Literature DB >> 9523239

Production and characterization of a monoclonal antibody against mannose-sensitive hemagglutinin of Vibrio cholerae.

G Falero1, B L Rodríguez, T Valmaseda, M E Pérez, J L Pérez, R Fando, A Robert, J Campos, A Silva, G Sierra, J A Benítez.   

Abstract

We have generated murine monoclonal antibodies (MAb) against Vibrio cholerae mannose-sensitive hemagglutinin (MSHA) using conventional hybridoma procedures. Seven hybridomas were obtained and one characterized. Hybridoma 2F12/F1 secreted an antibody of the IgG3 type that reacted with a 17-kDa antigen corresponding to the product of the mshA gene. This MAb inhibited mannose-sensitive agglutination of chicken erythrocytes by EL tor and O139 vibrios. Vibrios expressing MSHA activity inhibited binding of the antibody secreted by 2F12/F1 to MSHA-coated microtiter plates.

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Year:  1998        PMID： 9523239     DOI： 10.1089/hyb.1998.17.63

Source DB:  PubMed          Journal:  Hybridoma        ISSN： 0272-457X


  4 in total

1.  The Vibrio cholerae mannose-sensitive hemagglutinin is the receptor for a filamentous bacteriophage from V. cholerae O139.

Authors:  E A Jouravleva; G A McDonald; J W Marsh; R K Taylor; M Boesman-Finkelstein; R A Finkelstein
Journal:  Infect Immun       Date:  1998-06       Impact factor: 3.441

2.  VGJ phi, a novel filamentous phage of Vibrio cholerae, integrates into the same chromosomal site as CTX phi.

Authors:  Javier Campos; Eriel Martínez; Edith Suzarte; Boris L Rodríguez; Karen Marrero; Yussuan Silva; Talena Ledón; Ricardo del Sol; Rafael Fando
Journal:  J Bacteriol       Date:  2003-10       Impact factor: 3.490

3.  Preliminary assessment of the safety and immunogenicity of a new CTXPhi-negative, hemagglutinin/protease-defective El Tor strain as a cholera vaccine candidate.

Authors:  J A Benítez; L García; A Silva; H García; R Fando; B Cedré; A Pérez; J Campos; B L Rodríguez; J L Pérez; T Valmaseda; O Pérez; A Pérez; M Ramírez; T Ledón; M D Jidy; M Lastre; L Bravo; G Sierra
Journal:  Infect Immun       Date:  1999-02       Impact factor: 3.441

4.  Novel type of specialized transduction for CTX phi or its satellite phage RS1 mediated by filamentous phage VGJ phi in Vibrio cholerae.

Authors:  Javier Campos; Eriel Martínez; Karen Marrero; Yussuan Silva; Boris L Rodríguez; Edith Suzarte; Talena Ledón; Rafael Fando
Journal:  J Bacteriol       Date:  2003-12       Impact factor: 3.490
  4 in total

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