Comparison of sample source (excreta or ileal digesta) and age of broiler chick on measurement of apparent digestible energy of wheat and barley.

The broiler chick bioassay measures AME of wheat- or barley-based diets, with or without an enzyme, from excreta (24-h collections at 8 or 16 d) and ileal digesta (17 d). The objective was to discuss the merits and accuracy of sample source (excreta vs ileal digesta) and bird age for determining the feeding value of wheat and barley. The bioassay utilized 80% of a test cereal grain, 20% basal diet containing 1.1% acid insoluble ash marker, and fed with or without an enzyme to four pens of six male broilers from 4 to 17 d. A total of 138 wheat and 97 barley samples (with and without an enzyme) were tested in 15 and five bioassays, respectively. Within each wheat or barley bioassay two control wheat and barley samples were measured. The among-pens and between-assays CV for AME were calculated for these control samples, and correlation coefficients between the measures were calculated for the controls and for all of the 138 wheat and 97 barley samples included in the assays. For wheat samples, values for AME were lowest for excreta samples collected at 8 d, and similar for excreta and ileal digesta samples collected at 16 and 17 d, respectively. For barley samples, the three values were significantly different. The among-pens and between-assay CV were low for AME among both wheat and barley samples. Correlation coefficients between several measures of AME at 8 and 16 d were significant for the control samples with enzyme supplementation. When all samples were included in the analysis, correlation coefficients between AME measures were moderate to high. On the basis of accuracy, precision, and cost, these data favor measuring AME on excreta samples at 16 d of age. Comparisons of number of pens of broilers used to determine AME would suggest that much of the variability predicted with four pens of six broilers each could be achieved with three, and possibly two pens of six broilers each, thereby greatly increasing the capacity of the assay to screen large numbers of samples.