Literature DB >> 9519482

An improved colony-PCR method for filamentous fungi for amplification of PCR-fragments of several kilobases.

C M van Zeijl1, E H van de Kamp, P J Punt, G C Selten, B Hauer, R F van Gorcom, C A van den Hondel.   

Abstract

A method was developed to perform PCR directly on mycelial pellets or colonies treated with NOVOzym 234. The method allows rapid screening of large numbers of transformants of both sporulating and non-sporulating fungi for the presence of (co)transforming plasmid copies or for specific genetic modifications such as gene disruption and site specific integration. PCR fragments of at least 3.2 kb can be obtained. Using this method the identification of specific disruption mutants from Aspergillus niger and Beauveria bassiana was carried out.

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Year:  1997        PMID： 9519482     DOI： 10.1016/s0168-1656(97)00170-3

Source DB:  PubMed          Journal:  J Biotechnol        ISSN： 0168-1656            Impact factor:   3.307


  15 in total

1.  Characterization of a foldase, protein disulfide isomerase A, in the protein secretory pathway of Aspergillus niger.

Authors:  C Ngiam; D J Jeenes; P J Punt; C A Van Den Hondel; D B Archer
Journal:  Appl Environ Microbiol       Date:  2000-02       Impact factor: 4.792

2.  Fungal endophytic communities in grapevines (Vitis vinifera L.) respond to crop management.

Authors:  Michael Pancher; Marco Ceol; Paola Elisa Corneo; Claudia Maria Oliveira Longa; Sohail Yousaf; Ilaria Pertot; Andrea Campisano
Journal:  Appl Environ Microbiol       Date:  2012-04-06       Impact factor: 4.792

3.  CAP1, an adenylate cyclase-associated protein gene, regulates bud-hypha transitions, filamentous growth, and cyclic AMP levels and is required for virulence of Candida albicans.

Authors:  Y S Bahn; P Sundstrom
Journal:  J Bacteriol       Date:  2001-05       Impact factor: 3.490

4.  Identification of a fungal 1,8-cineole synthase from Hypoxylon sp. with specificity determinants in common with the plant synthases.

Authors:  Jeffrey J Shaw; Tetyana Berbasova; Tomoaki Sasaki; Kyra Jefferson-George; Daniel J Spakowicz; Brian F Dunican; Carolina E Portero; Alexandra Narváez-Trujillo; Scott A Strobel
Journal:  J Biol Chem       Date:  2015-02-03       Impact factor: 5.157

5.  Calnexin overexpression increases manganese peroxidase production in Aspergillus niger.

Authors:  Ana Conesa; David Jeenes; David B Archer; Cees A M J J van den Hondel; Peter J Punt
Journal:  Appl Environ Microbiol       Date:  2002-02       Impact factor: 4.792

6.  Molecular approach for the rapid detection of Bacillus and Pseudomonas genera--dominant antagonistic groups--from diverse ecological niches using colony multiplex PCR.

Authors:  Anusree V Nair; M A Pradeep; K K Vijayan
Journal:  J Ind Microbiol Biotechnol       Date:  2014-04-22       Impact factor: 3.346

7.  Studies on the production of fungal peroxidases in Aspergillus niger.

Authors:  A Conesa; C A van den Hondel; P J Punt
Journal:  Appl Environ Microbiol       Date:  2000-07       Impact factor: 4.792

8.  Isolation of Taxol-Producing Endophytic Fungi from Iranian Yew Through Novel Molecular Approach and Their Effects on Human Breast Cancer Cell Line.

Authors:  Abdollah Kasaei; Mohsen Mobini-Dehkordi; Foruzandeh Mahjoubi; Behnaz Saffar
Journal:  Curr Microbiol       Date:  2017-03-23       Impact factor: 2.188

9.  Function of the cypX and moxY genes in aflatoxin biosynthesis in Aspergillus parasiticus.

Authors:  Ying Wen; Hidemi Hatabayashi; Hatsue Arai; Hiroko K Kitamoto; Kimiko Yabe
Journal:  Appl Environ Microbiol       Date:  2005-06       Impact factor: 4.792

10.  Genome of Diaporthe sp. provides insights into the potential inter-phylum transfer of a fungal sesquiterpenoid biosynthetic pathway.

Authors:  Jose Guedes de Sena Filho; Maureen B Quin; Daniel J Spakowicz; Jeffrey J Shaw; Kaury Kucera; Brian Dunican; Scott A Strobel; Claudia Schmidt-Dannert
Journal:  Fungal Biol       Date:  2016-04-12
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