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Literature DB >> 9515968

The hyperthermophile chromosomal protein Sac7d sharply kinks DNA.

H Robinson¹, Y G Gao, B S McCrary, S P Edmondson, J W Shriver, A H Wang.

Abstract

The proteins Sac7d and Sso7d belong to a class of small chromosomal proteins from the hyperthermophilic archaeon Sulfolobus acidocaldarius and S. solfactaricus, respectively. These proteins are extremely stable to heat, acid and chemical agents. Sac7d binds to DNA without any particular sequence preference and thereby increases its melting temperature by approximately 40 degrees C. We have now solved and refined the crystal structure of Sac7d in complex with two DNA sequences to high resolution. The structures are examples of a nonspecific DNA-binding protein bound to DNA, and reveal that Sac7d binds in the minor groove, causing a sharp kinking of the DNA helix that is more marked than that induced by any sequence-specific DNA-binding proteins. The kink results from the intercalation of specific hydrophobic side chains of Sac7d into the DNA structure, but without causing any significant distortion of the protein structure relative to the uncomplexed protein in solution.

Entities: Gene Species

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Year: 1998 PMID： 9515968 DOI： 10.1038/32455

Source DB: PubMed Journal: Nature ISSN： 0028-0836 Impact factor: 49.962

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Cited

57 in total

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Journal: Proc Natl Acad Sci U S A Date: 2000-07-18 Impact factor: 11.205

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Journal: Proc Natl Acad Sci U S A Date: 2001-06-26 Impact factor: 11.205

3. Molecular determinants for targeting heterochromatin protein 1-mediated gene silencing: direct chromoshadow domain-KAP-1 corepressor interaction is essential.

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4. Nonspecific DNA binding and bending by HUαβ: interfaces of the three binding modes characterized by salt-dependent thermodynamics.

Authors: Junseock Koh; Irina Shkel; Ruth M Saecker; M Thomas Record
Journal: J Mol Biol Date: 2011-04-12 Impact factor: 5.469

The hyperthermophile chromosomal protein Sac7d sharply kinks DNA.

1. Crystal structure of the HIV-1 integrase catalytic core and C-terminal domains: a model for viral DNA binding.

2. The complete genome of the crenarchaeon Sulfolobus solfataricus P2.

3. Molecular determinants for targeting heterochromatin protein 1-mediated gene silencing: direct chromoshadow domain-KAP-1 corepressor interaction is essential.

4. Nonspecific DNA binding and bending by HUαβ: interfaces of the three binding modes characterized by salt-dependent thermodynamics.

5. Crystal structure of a DNA binding protein from the hyperthermophilic euryarchaeon Methanococcus jannaschii.

6. Recognition of DNA substrates by T4 bacteriophage polynucleotide kinase.

7. Crystal structure of the hyperthermophilic archaeal DNA-binding protein Sso10b2 at a resolution of 1.85 Angstroms.

8. DNA binding and cleavage by the periplasmic nuclease Vvn: a novel structure with a known active site.

9. Crystal structure of archaeal chromatin protein Alba2-double-stranded DNA complex from Aeropyrum pernix K1.

10. Crystal structure of the crenarchaeal conserved chromatin protein Cren7 and double-stranded DNA complex.