Literature DB >> 9512096

Molecular cloning and expression of cDNA for human RNase H.

H Wu1, W F Lima, S T Crooke.   

Abstract

We have cloned, expressed, and purified to electrophoretic homogeneity a human RNase H. The enzyme has a molecular weight of 32 kDa, is Mg2+ dependent, and is inhibited by Mn2+ and N-ethylmaleimide. Its molecular weight and cleavage characteristics are consistent with type 2 human RNase H. The human RNase H we have cloned is highly homologous to Escherichia coli RNase HI (33.6% amino acid identity) and to other RNase H enzymes homologous to E. coli RNase HI. The enzyme is encoded by a single gene that is at least 10 kb in length and is expressed ubiquitously in human cells and tissues.

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Year:  1998        PMID： 9512096     DOI： 10.1089/oli.1.1998.8.53

Source DB:  PubMed          Journal:  Antisense Nucleic Acid Drug Dev        ISSN： 1087-2906


  10 in total

1.  An upstream open reading frame and the context of the two AUG codons affect the abundance of mitochondrial and nuclear RNase H1.

Authors:  Yutaka Suzuki; J Bradley Holmes; Susana M Cerritelli; Kiran Sakhuja; Michal Minczuk; Ian J Holt; Robert J Crouch
Journal:  Mol Cell Biol       Date:  2010-09-07       Impact factor: 4.272

2.  Increased efficacy of antileishmanial antisense phosphorothioate oligonucleotides in Leishmania amazonensis overexpressing ribonuclease H.

Authors:  M Mishra; J R Bennett; G Chaudhuri
Journal:  Biochem Pharmacol       Date:  2001-02-15       Impact factor: 5.858

Review 3.  Antisense technology: A review.

Authors:  Stanley T Crooke; Xue-Hai Liang; Brenda F Baker; Rosanne M Crooke
Journal:  J Biol Chem       Date:  2021-02-16       Impact factor: 5.157

4.  Gene cloning and characterization of recombinant RNase HII from a hyperthermophilic archaeon.

Authors:  M Haruki; K Hayashi; T Kochi; A Muroya; Y Koga; M Morikawa; T Imanaka; S Kanaya
Journal:  J Bacteriol       Date:  1998-12       Impact factor: 3.490

5.  Selective inhibitory DNA aptamers of the human RNase H1.

Authors:  Frédéric Pileur; Marie-Line Andreola; Eric Dausse; Justine Michel; Serge Moreau; Hirofumi Yamada; Sergei A Gaidamakov; Robert J Crouch; Jean-Jacques Toulmé; Christian Cazenave
Journal:  Nucleic Acids Res       Date:  2003-10-01       Impact factor: 16.971

6.  Circularized synthetic oligodeoxynucleotides serve as promoterless RNA polymerase III templates for small RNA generation in human cells.

Authors:  Christine I Seidl; Lodoe Lama; Kevin Ryan
Journal:  Nucleic Acids Res       Date:  2012-12-28       Impact factor: 16.971

7.  Human RNase H1 is associated with protein P32 and is involved in mitochondrial pre-rRNA processing.

Authors:  Hongjiang Wu; Hong Sun; Xuehai Liang; Walt F Lima; Stanley T Crooke
Journal:  PLoS One       Date:  2013-08-22       Impact factor: 3.240

Review 8.  Molecular Mechanisms of Antisense Oligonucleotides.

Authors:  Stanley T Crooke
Journal:  Nucleic Acid Ther       Date:  2017-01-12       Impact factor: 5.486

9.  Viable RNaseH1 knockout mice show RNaseH1 is essential for R loop processing, mitochondrial and liver function.

Authors:  Walt F Lima; Heather M Murray; Sagar S Damle; Christopher E Hart; Gene Hung; Cheryl Li De Hoyos; Xue-Hai Liang; Stanley T Crooke
Journal:  Nucleic Acids Res       Date:  2016-04-29       Impact factor: 16.971

10.  Influence of mismatched and bulged nucleotides on SNP-preferential RNase H cleavage of RNA-antisense gapmer heteroduplexes.

Authors:  Dorota Magner; Ewa Biala; Jolanta Lisowiec-Wachnicka; Ryszard Kierzek
Journal:  Sci Rep       Date:  2017-10-02       Impact factor: 4.379
  10 in total

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