| Literature DB >> 9511828 |
L M Dalla-Costa1, K Irino, J Rodrigues, I N Rivera, L R Trabulsi.
Abstract
The ability of ribotyping and enterobacterial repetitive intergenic concensus-polymerase chain reaction (ERIC-PCR) to discriminate diarrhoeagenic Escherichia coli clones of 122 strains belonging to 26 distinct serotypes was evaluated. The 26 serotypes corresponded to 24 ribotypes and 25 ERIC-types. Correlation between multilocus enzyme electrophoresis, ERIC-PCR and ribotyping was c. 90% for the dominant ribotypes. Related clones such as O55:H7 and O157:H7 presented similar ribotypes and clustered together in a dendrogram, and the two divergent clonal groups of enteropathogenic E. coli (EPEC) and enterohaemorrhagic E. coli (EHEC) were included in distinct branches. The results suggest the possibility of applying these two simpler techniques as tools to identify clones of diarrhoeagenic E. coli.Entities:
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Year: 1998 PMID： 9511828 DOI： 10.1099/00222615-47-3-227
Source DB: PubMed Journal: J Med Microbiol ISSN： 0022-2615 Impact factor: 2.472