Physiological and pathological factors of human breast disease that can influence optical diagnosis.

The 'normal' human female breast is a very complex organ that changes considerably during development, pregnancy and menopause. In addition, it is an excretory organ that, during lactation, discharges various metabolites and certain drugs that can be optically active. Optical diagnosis of breast cancers requires detection of differential concentrations of 1) various absorbers and scatterers or 2) native or exogenous fluorophores to distinguish cancers from surrounding 'normal' and benign breast tissues. The differential concentrations are due to the biology of the cancer cells and the host responses to the cancer growth. For most patients, the cancer will be intermixed with a complex 3-dimensional array of 'normal' breast tissue and benign breast lesions. This complexity will challenge the optical biopsy investigator but, with the recent advances in our understanding of light transport, optical diagnostic techniques and devices can be developed to complement and supplement current breast cancer screening techniques.