| Literature DB >> 9501530 |
M Kataoka1, L P Sri Rohani, M Wada, K Kita, H Yanase, I Urabe, S Shimizu.
Abstract
Escherichia coli JM109 (pGDA2) overexpressing the glucose dehydrogenase (GDH) gene from Bacillus megaterium IWG3 was examined for use as a cofactor regenerator. In the asymmetric reduction of ethyl 4-chloro-3-oxobutanoate by E. coli JM109 (pKAR) which is an aldehyde reductase-overproducing transformant, E. coli JM109 (pGDA2) can act as an NADPH regenerator with NADP+ and glucose, similarly to commercially available GDH.Entities:
Mesh:
Substances:
Year: 1998 PMID: 9501530 DOI: 10.1271/bbb.62.167
Source DB: PubMed Journal: Biosci Biotechnol Biochem ISSN: 0916-8451 Impact factor: 2.043