Literature DB >> 950006

Affinity chromatography of soluble galactosyltransferases using an easily synthesized N-acetylglucosamine-agarose bead.

E G Berger, M M Weiser, K J Isselbacher.   

Abstract

Agarose derivatized wtih phi-nitrophenyl-N-acetyl-beta-d-glucosamine was used for affinity chromatography for soluble galactosyltransferase from various sources. Moreover, this adsorbant acts as an acceptor for galactose when incubated with prepurified galactosyltransferase.

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Year:  1976        PMID: 950006     DOI: 10.1007/bf01919834

Source DB:  PubMed          Journal:  Experientia        ISSN: 0014-4754


  7 in total

1.  Protein measurement with the Folin phenol reagent.

Authors:  O H LOWRY; N J ROSEBROUGH; A L FARR; R J RANDALL
Journal:  J Biol Chem       Date:  1951-11       Impact factor: 5.157

2.  Studies on the oligosaccharide sequence of ribonuclease B.

Authors:  A Tarentino; T H Plummer; F Maley
Journal:  J Biol Chem       Date:  1970-08-25       Impact factor: 5.157

3.  A rapid procedure for derivatizing agarose with a variety of carbohydrates: its use for affinity chromatography of lectins.

Authors:  R Bloch; M M Burger
Journal:  FEBS Lett       Date:  1974-08-30       Impact factor: 4.124

4.  Agarose derivatives of uridine diphosphate and N-acetylglucosamine for the purification of a galactosyltransferase.

Authors:  R Barker; K W Olsen; J H Shaper; R L Hill
Journal:  J Biol Chem       Date:  1972-11-25       Impact factor: 5.157

5.  The purification and properties of the A protein of lactose synthetase.

Authors:  I P Trayer; R L Hill
Journal:  J Biol Chem       Date:  1971-11       Impact factor: 5.157

6.  Role of cell membrane galactosyltransferase in concanavalin A agglutination of erythrocytes.

Authors:  D K Podolsky; M M Weiser
Journal:  Biochem J       Date:  1975-01       Impact factor: 3.857

7.  Glycosyltransferases in human blood. II. Study of serum galactosyltransferase and N-acetylgalactosaminyltransferase in patients with liver diseases.

Authors:  Y S Kim; J Perdomo; J S Whitehead; K J Curtis
Journal:  J Clin Invest       Date:  1972-08       Impact factor: 14.808

  7 in total

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