Literature DB >> 949330

Removal of adsorbed elastase from partially digested elastin.

H P Fromageot, G M Slusarczuk.   

Abstract

Bovine ligamentum nuchae elastin that had been partially digested by porcine pancreatic elastase and then thoroughly washed with 0.2 M-NaC1/0.05 M-sodium borate solution continued to release peptide fragments in solution. Complete removal of elastase from the elastin surface required extraction with dilute alkali, a procedure that does not irreversibly inactivate the enzyme or hydrolyse elastin.

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Year:  1976        PMID: 949330      PMCID: PMC1172896          DOI: 10.1042/bj1550705

Source DB:  PubMed          Journal:  Biochem J        ISSN: 0264-6021            Impact factor:   3.857


  7 in total

1.  Photometric method for estimation of elastase activity.

Authors:  L A SACHAR; K K WINTER; N SICHER; S FRANKEL
Journal:  Proc Soc Exp Biol Med       Date:  1955-11

2.  Studies on porcine elastase and proelastase.

Authors:  N H GRANT; K C ROBBINS
Journal:  Arch Biochem Biophys       Date:  1957-02       Impact factor: 4.013

3.  Pancreatic elastase: purification, properties, and function.

Authors:  N G BRINK; U J LEWIS; D E WILLIAMS
Journal:  J Biol Chem       Date:  1956-10       Impact factor: 5.157

4.  The structure and chemical characterization of elastic fibers as revealed by elastase and by electron microscopy.

Authors:  A I LANSING; T B ROSENTHAL; M ALEX; E W DEMPSEY
Journal:  Anat Rec       Date:  1952-12

5.  The non-specific electrostatic nature of the adsorption of elastase and other basic proteins on elastin.

Authors:  A Gertler
Journal:  Eur J Biochem       Date:  1971-06-29

6.  Isolation and characterization of Clostridium botulinum type B toxin.

Authors:  W H Beers; E Reich
Journal:  J Biol Chem       Date:  1969-08-25       Impact factor: 5.157

7.  The protein and lipid composition of arterial elastin and its relationship to lipid accumulation in the atherosclerotic plaque.

Authors:  D M Kramsch; C Franzblau; W Hollander
Journal:  J Clin Invest       Date:  1971-08       Impact factor: 14.808

  7 in total

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