The development of antibody responses in the infant.

The human infant is a major subject of immunization to prevent infectious disease. A better understanding of immune responses in human infants could lead to more effective immunization. Analysis of individual mechanisms is a prerequisite to understanding the whole response. We have examined the mutation and selection of immunoglobulin genes, mechanisms required for the maturation of antibody affinity. Mutations were rare in cord blood and were not detected in peripheral blood of infants under 10 days of age. Mutations were detected in most blood samples from infants aged 10 days to 2 months but the number of mutations was generally low compared with adults and there was limited evidence of selection. The ability to make and select mutated Ig increased over the 2-8 months period, but did not reach adult levels. Neonates differ from adults in the expression of several components of the immune system that may take part in affinity maturation, including cytokine receptors, antigen receptor and co-stimulator molecules. Thus, the mechanisms required to make high-affinity antibody develop gradually during the first year of life. Possible evolutionary reasons for this are discussed.