Literature DB >> 94918

Rapid isolation of carcinogen-bound DNA and RNA by hydroxyapatite chromatography.

F A Beland, K L Dooley, D A Casciano.   

Abstract

Carcinogen-bound DNA and RNA are conveniently isolated by solvent extraction and hydroxyapatite (HAP) chromatography. Tissue is suspended in 8 M urea-0.24 M sodium phosphate-1% sodium dodecyl sulfate-10 mM EDTA, pH 6.8 (MUP-SDS-EDTA) and extracted with chloroform-isoamyl alcohol-phenol (24:1:25; CIP) to remove protein. RNA and DNA are separated by passing the aqueous solution through an HAP column; RNA is eluted with MUP, DNA with 0.48 M sodium phosphate, pH 6.8. Examples presented are: (1) calf thymus DNA that has been reacted with N-acetoxy-2-acetylaminofluorene (N-OAc-AAF), (2) isolated intact rat hepatocytes incubated with N-hydroxy-AAF and (3) livers from Sprague-Dawley rats treated with N-hydroxy-AAF.

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Year:  1979        PMID： 94918     DOI： 10.1016/s0021-9673(00)87048-x

Source DB:  PubMed          Journal:  J Chromatogr


  15 in total

1.  Evidence for the involvement of a nitrenium ion in the covalent binding of nitrofurazone to DNA.

Authors:  A J Streeter; B A Hoener
Journal:  Pharm Res       Date:  1988-07       Impact factor: 4.200

2.  DNA base changes induced following in vivo exposure of unadapted, adapted or ada- Escherichia coli to N-methyl-N'-nitro-N-nitrosoguanidine.

Authors:  K K Richardson; R M Crosby; F C Richardson; T R Skopek
Journal:  Mol Gen Genet       Date:  1987-10

3.  Increased 8-hydroxyguanine content of chloroplast DNA from ozone-treated plants.

Authors:  R A Floyd; M S West; W E Hogsett; D T Tingey
Journal:  Plant Physiol       Date:  1989-10       Impact factor: 8.340

4.  DNA base changes and alkylation following in vivo exposure of Escherichia coli to N-methyl-N-nitrosourea or N-ethyl-N-nitrosourea.

Authors:  K K Richardson; F C Richardson; R M Crosby; J A Swenberg; T R Skopek
Journal:  Proc Natl Acad Sci U S A       Date:  1987-01       Impact factor: 11.205

5.  O4-ethyldeoxythymidine, but not O6-ethyldeoxyguanosine, accumulates in hepatocyte DNA of rats exposed continuously to diethylnitrosamine.

Authors:  J A Swenberg; M C Dyroff; M A Bedell; J A Popp; N Huh; U Kirstein; M F Rajewsky
Journal:  Proc Natl Acad Sci U S A       Date:  1984-03       Impact factor: 11.205

6.  Reactivity of B and Z-DNA towards N-acetoxy-2-acetylaminofluorene.

Authors:  M Spodheim-Maurizot; B Malfoy; G Saint-Ruf
Journal:  Nucleic Acids Res       Date:  1982-07-24       Impact factor: 16.971

7.  Aminofluorene-DNA adduct formation in Salmonella typhimurium exposed to the carcinogen N-hydroxy-2-acetylaminofluorene.

Authors:  D T Beranek; G L White; R H Heflich; F A Beland
Journal:  Proc Natl Acad Sci U S A       Date:  1982-09       Impact factor: 11.205

8.  Arylamine-DNA adducts in vitro and in vivo: their role in bacterial mutagenesis and urinary bladder carcinogenesis.

Authors:  F A Beland; D T Beranek; K L Dooley; R H Heflich; F F Kadlubar
Journal:  Environ Health Perspect       Date:  1983-03       Impact factor: 9.031

9.  Metabolic activation routes of arylamines and their genotoxic effects.

Authors:  J H Meerman; M L van de Poll
Journal:  Environ Health Perspect       Date:  1994-10       Impact factor: 9.031

10.  Formation and persistence of arylamine DNA adducts in vivo.

Authors:  F A Beland; F F Kadlubar
Journal:  Environ Health Perspect       Date:  1985-10       Impact factor: 9.031
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