Neuromuscular specializations of the pharyngeal dilator muscles: I. Compartments of the canine geniohyoid muscle.

BACKGROUND: Little is known about the structure and innervation of the geniohyoid muscle (GH), which is an important pharyngeal dilator muscle activated in swallowing and respiration.
METHODS: The neuromuscular specializations of the canine GH were studied in detail by using a combination of histological, histochemical, and anatomical techniques. First, hematoxylin and eosin staining, Gomori's rapid one-step trichrome stain, and silver impregnation were used to determine the terminations of muscle fibers and existence of fibrous septa within the muscle (n = 8). Second, myofibrillar ATPase staining was employed to document the muscle fiber type distribution (n = 8). Finally, Sihler's stain (n = 10) and wholemount acetylcholinesterase staining (n = 8) were used to examine the distribution of the nerve supply within the muscle.
RESULTS: The canine GH is divided into rostral and caudal compartments, which are arranged in series and separated by a transverse fibrous septum. Each compartment receives its own primary nerve branch, which supplies a separate motor endplate zone. The rostral compartment is innervated bilaterally, whereas the caudal compartment is innervated ipsilaterally. The rostral compartment was composed of significantly more type I (slow twitch) muscle fibers (56%) than the caudal compartment (25%).
CONCLUSIONS: The canine GH is composed of two in-series neuromuscular compartments rather than a single muscle as traditionally believed. This anatomical finding suggests that these two compartments may function independently under different physiological conditions.