Mutations of the CDC28 gene and the radiation sensitivity of Saccharomyces cerevisiae.

cdc28-srm, a non-temperature-sensitive (ts) mutation in the CDC28 gene of Saccharomyces cerevisiae that affects fidelity of mitotic transmission of both mitochondrial and nuclear genetic structures (Devin et al., 1990), also affected cell growth and sensitivity to lethal effects of ionizing radiation. At 30 degrees C cdc28-13, a ts mutation, was without appreciable effects on spontaneous mitochondrial rho(-)-mutagenesis, cell growth and radiation sensitivity, whereas all three cell characteristics mentioned were affected (although to a lesser degree than by cdc28-srm) by cdc28-1, another ts mutation. cdc28-srm was without any significant effect on the rates of spontaneous nuclear gene mutations and gamma-ray-induced mitotic recombination. An analysis of double mutants as regards their radiation sensitivity has revealed additive or even synergistic interactions between the cdc28-srm mutation and every one of the rad6-1 and rad52-1 mutations. The rad9 delta allele was found to be epistatic to cdc28-srm. These data suggest that the p34CDC28 protein is involved in the RAD9-dependent feedback control of DNA integrity operating at the cell cycle checkpoints.