Literature DB >> 9483798

Efficient PCR-based random mutagenesis of sub-genic (100 bp) DNA fragments.

V Svetlov1, T G Cooper.   

Abstract

Here we describe a method for performing a PCR-driven random mutagenesis of 100 bp DNA fragments that yields mutations at a useful frequency. The method is a modification of the manganese ion substitution PCR technique, and neither creates 'hot-spots' nor favors transition mutations over transversions.

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Year:  1998        PMID： 9483798     DOI： 10.1002/(SICI)1097-0061(19980115)14:1<89::AID-YEA194>3.0.CO;2-3

Source DB:  PubMed          Journal:  Yeast        ISSN： 0749-503X            Impact factor:   3.239


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