| Literature DB >> 9481773 |
Abstract
The effect of digestion in trypsin and acid pepsin on Toxoplasma gondii tachyzoites and bradyzoites was re-evaluated because of recent use of this method to distinguish tachyzoites from bradyzoites. Toxoplasma gondii tachyzoites survived better in 0.5% trypsin solution for 1 h than in 1.0% solution, and occasionally survived for 2 h in acid pepsin solution. Extracellular tachyzoites (> or = 1000) were also infectious orally to mice and cats. Bradyzoites survived equally in trypsin and acid pepsin solutions but the digestion of brain tissue in 0.5% trypsin solution was better than in acid pepsin solution. The resistance to digestion in acid pepsin solution is not a reliable method to distinguish tachyzoites from bradyzoites.Entities:
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Year: 1998 PMID： 9481773 DOI： 10.1017/s0031182097001935
Source DB: PubMed Journal: Parasitology ISSN： 0031-1820 Impact factor: 3.234