Literature DB >> 9473152

A novel method to assay herpes simplex virus neutralizing antibodies using BHKICP6LacZ-5 (ELVIS) cells.

R L Ashley1, J Dalessio, R E Sekulovich.   

Abstract

A novel method for determining neutralizing serum antibody titers to herpes simplex virus type 2 (HSV-2) was developed based on reduction of infectivity in BHKICP6LacZ-5 (ELVIS) cells; baby hamster kidney (BHK) cells that have been genetically engineered to contain the Escherichia coli LacZ gene under the control of an inducible herpes simplex virus type 1 (HSV-1) promoter. The test has a semiautomated, colorimetric readout resulting in rapid, objective readings of infectivity reduction. Extent of neutralization is calculated against a calibration curve of virus infectivity generated in each run. HSV-2 neutralizing activity can be detected with serum dilutions in excess of 1:5120.

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Year:  1997        PMID： 9473152     DOI： 10.1089/vim.1997.10.213

Source DB:  PubMed          Journal:  Viral Immunol        ISSN： 0882-8245            Impact factor:   2.257


  5 in total

1.  Confirmation of low-titer, herpes simplex virus-positive specimen results by the enzyme-linked virus-inducible system (ELVIS) using PCR and repeat testing.

Authors:  N Patel; L Kauffmann; G Baniewicz; M Forman; M Evans; D Scholl
Journal:  J Clin Microbiol       Date:  1999-12       Impact factor: 5.948

Review 2.  Role of cell culture for virus detection in the age of technology.

Authors:  Diane S Leland; Christine C Ginocchio
Journal:  Clin Microbiol Rev       Date:  2007-01       Impact factor: 26.132

3.  Cervicovaginal neutralizing antibodies to herpes simplex virus (HSV) in women seropositive for HSV Types 1 and 2.

Authors:  Francois-Xavier Mbopi-Kéou; Laurent Bélec; Julie Dalessio; Jérôme Legoff; Gérard Grésenguet; Philippe Mayaud; David W G Brown; Rhoda Ashley Morrow
Journal:  Clin Diagn Lab Immunol       Date:  2003-05

4.  Correlation between herpes simplex virus neutralizing antibody titers determined by ELVIS cell and traditional plaque reduction assays.

Authors:  Tamara P Blevins; Yinyi Yu; Robert B Belshe; Abbie R Bellamy; Lynda A Morrison
Journal:  PLoS One       Date:  2019-04-04       Impact factor: 3.240

5.  Higher Throughput Quantification of Neutralizing Antibody to Herpes Simplex Viruses.

Authors:  Tamara P Blevins; Michelle C Mitchell; Maria Korom; Hong Wang; Yinyi Yu; Lynda A Morrison; Robert B Belshe
Journal:  PLoS One       Date:  2015-12-14       Impact factor: 3.240
  5 in total

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