Literature DB >> 9473051

Biochemical and genetic characterization of trans-2'-carboxybenzalpyruvate hydratase-aldolase from a phenanthrene-degrading Nocardioides strain.

T Iwabuchi1, S Harayama.   

Abstract

trans-2'-Carboxybenzalpyruvate hydratase-aldolase was purified from a phenanthrene-degrading bacterium, Nocardioides sp. strain KP7, and characterized. The purified enzyme was found to have molecular masses of 38 kDa by sodium dodecyl sulfate-polyacrylamide gel electrophoresis and 113 kDa by gel filtration chromatography. Thus, the homotrimer of the 38-kDa subunit constituted an active enzyme. The Km and kcat values of this enzyme for trans-2'-carboxybenzalpyruvate were 50 microM and 13 s(-1), respectively. trans-2'-Carboxybenzalpyruvate was transformed to 2-carboxybenzaldehyde and pyruvate by the action of this enzyme. The structural gene for this enzyme was cloned and sequenced; the length of this gene was 996 bp. The deduced amino acid sequence of this enzyme exhibited homology to those of trans-2'-hydroxybenzalpyruvate hydratase-aldolases from Pseudomonas putida PpG7 and Pseudomonas sp. strain C18.

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Year:  1998        PMID： 9473051      PMCID： PMC106976     

Source DB:  PubMed          Journal:  J Bacteriol        ISSN： 0021-9193            Impact factor:   3.490


  21 in total

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2.  Cloning, nucleotide sequence and characterization of genes encoding naphthalene dioxygenase of Pseudomonas putida strain NCIB9816.

Authors:  S Kurkela; H Lehväslaiho; E T Palva; T H Teeri
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3.  Sequences of genes encoding naphthalene dioxygenase in Pseudomonas putida strains G7 and NCIB 9816-4.

Authors:  M J Simon; T D Osslund; R Saunders; B D Ensley; S Suggs; A Harcourt; W C Suen; D L Cruden; D T Gibson; G J Zylstra
Journal:  Gene       Date:  1993-05-15       Impact factor: 3.688

4.  Identification and characterization of genes encoding polycyclic aromatic hydrocarbon dioxygenase and polycyclic aromatic hydrocarbon dihydrodiol dehydrogenase in Pseudomonas putida OUS82.

Authors:  N Takizawa; N Kaida; S Torigoe; T Moritani; T Sawada; S Satoh; H Kiyohara
Journal:  J Bacteriol       Date:  1994-04       Impact factor: 3.490

5.  Purification and properties of 2'-hydroxybenzalpyruvate aldolase from a bacterium that degrades naphthalenesulfonates.

Authors:  A E Kuhm; H J Knackmuss; A Stolz
Journal:  J Biol Chem       Date:  1993-05-05       Impact factor: 5.157

6.  Metabolism of dibenzothiophene and naphthalene in Pseudomonas strains: complete DNA sequence of an upper naphthalene catabolic pathway.

Authors:  S A Denome; D C Stanley; E S Olson; K D Young
Journal:  J Bacteriol       Date:  1993-11       Impact factor: 3.490

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8.  Metabolism of naphthalene, fluorene, and phenanthrene: preliminary characterization of a cloned gene cluster from Pseudomonas putida NCIB 9816.

Authors:  Y Yang; R F Chen; M P Shiaris
Journal:  J Bacteriol       Date:  1994-04       Impact factor: 3.490

9.  Cloning and characterization of a chromosomal gene cluster, pah, that encodes the upper pathway for phenanthrene and naphthalene utilization by Pseudomonas putida OUS82.

Authors:  H Kiyohara; S Torigoe; N Kaida; T Asaki; T Iida; H Hayashi; N Takizawa
Journal:  J Bacteriol       Date:  1994-04       Impact factor: 3.490

10.  Bacterial metabolism of naphthalene: construction and use of recombinant bacteria to study ring cleavage of 1,2-dihydroxynaphthalene and subsequent reactions.

Authors:  R W Eaton; P J Chapman
Journal:  J Bacteriol       Date:  1992-12       Impact factor: 3.490
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  13 in total

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2.  Structural Characterization of the Hydratase-Aldolases, NahE and PhdJ: Implications for the Specificity, Catalysis, and N-Acetylneuraminate Lyase Subgroup of the Aldolase Superfamily.

Authors:  Jake A LeVieux; Brenda Medellin; William H Johnson; Kaci Erwin; Wenzong Li; Ingrid A Johnson; Yan Jessie Zhang; Christian P Whitman
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3.  A novel phenanthrene dioxygenase from Nocardioides sp. Strain KP7: expression in Escherichia coli.

Authors:  A Saito; T Iwabuchi; S Harayama
Journal:  J Bacteriol       Date:  2000-04       Impact factor: 3.490

4.  Impact of irradiation and polycyclic aromatic hydrocarbon spiking on microbial populations in marine sediment for future aging and biodegradability studies.

Authors:  Rebecca J Melcher; Sabine E Apitz; Barbara B Hemmingsen
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5.  Structural basis for substrate specificity and mechanism of N-acetyl-D-neuraminic acid lyase from Pasteurella multocida.

Authors:  Nhung Huynh; Aye Aye; Yanhong Li; Hai Yu; Hongzhi Cao; Vinod Kumar Tiwari; Don-Wook Shin; Xi Chen; Andrew J Fisher
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6.  Mycobacterium aromativorans JS19b1(T) Degrades Phenanthrene through C-1,2, C-3,4 and C-9,10 Dioxygenation Pathways.

Authors:  Jong-Su Seo; Young-Soo Keum; Qing X Li
Journal:  Int Biodeterior Biodegradation       Date:  2012-03-21       Impact factor: 4.320

7.  Molecular characterization and substrate preference of a polycyclic aromatic hydrocarbon dioxygenase from Cycloclasticus sp. strain A5.

Authors:  Yuki Kasai; Kazutoshi Shindo; Shigeaki Harayama; Norihiko Misawa
Journal:  Appl Environ Microbiol       Date:  2003-11       Impact factor: 4.792

8.  Identification of pyrene-induced proteins in Mycobacterium sp. strain 6PY1: evidence for two ring-hydroxylating dioxygenases.

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Journal:  J Bacteriol       Date:  2003-07       Impact factor: 3.490

9.  Structure of the ring cleavage product of 1-hydroxy-2-naphthoate, an intermediate of the phenanthrene-degradative pathway of Nocardioides sp. strain KP7.

Authors:  K Adachi; T Iwabuchi; H Sano; S Harayama
Journal:  J Bacteriol       Date:  1999-02       Impact factor: 3.490

10.  Cloning, expression, purification, crystallization and preliminary X-ray diffraction studies of N-acetylneuraminate lyase from methicillin-resistant Staphylococcus aureus.

Authors:  Rachel A North; Sarah A Kessans; Sarah C Atkinson; Hironori Suzuki; Andrew J A Watson; Benjamin R Burgess; Lauren M Angley; André O Hudson; Arvind Varsani; Michael D W Griffin; Antony J Fairbanks; Renwick C J Dobson
Journal:  Acta Crystallogr Sect F Struct Biol Cryst Commun       Date:  2013-02-27
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