Comparison between the direct capillary tube leucocyte migration technique (LMCT) and the direct agarose leucocyte migration technique (LMAT) as indicators of tumour-directed cell-mediated hypersensitivity (TCMH) in patients with renal carcinoma.

A comparison was made between the direct capillary tube leucocyte migration technique (LMCT) and the direct agarose leucocyte migration technique (LMAT) using crude homogenates of tumour tissue as antigen in patients with renal carcinoma. A state of specifically altered reactivity of leucocytes from patients with renal carcinoma as compared with normal control persons towards allogeneic hypernephroma extracts could be detected in the LMCT but not in the LMAT. The reactivity in the LMCT was manifest at protein concentrations of 300 and 400 mug/ml. The study indicates that in contrast to the LMCT, the LMAT cannot be used for detection of TCMH towards allogeneic hypernephroma tissue extracts in patients with renal carcinoma.