PURPOSE: In an effort to show the differences between neurotrophic factors, laminin and acidic fibroblast growth factor (aFGF) were compared in terms of their abilities to regenerate axons in vivo over an extended distance. MATERIALS AND METHODS: The sciatic nerve was transected in 15 Sprague-Dawley rats. A 15-mm Silastic tube (Dow Corning, Midland, MI) was placed between the ends of the cut nerve and filled with either laminin, aFGF, or buffer applied to collagen sponges. RESULTS: Ten weeks postimplantation, mean axon counts showed that both laminin (2432) and aFGF (1612) produced significantly higher numbers of axons than controls (1009) (P < .05) and that laminin showed significantly more nerve regeneration than aFGF (P < .05). CONCLUSION: These results indicate that laminin and aFGF enhance peripheral nerve regeneration across a large gap, presumably through their neurotrophic effects and mitogenic properties on supporting cells. Furthermore, it is concluded that the transient nature of aFGF's effect on the regenerative environment limits its effectiveness at regenerating axons over a prolonged period of time.
PURPOSE: In an effort to show the differences between neurotrophic factors, laminin and acidic fibroblast growth factor (aFGF) were compared in terms of their abilities to regenerate axons in vivo over an extended distance. MATERIALS AND METHODS: The sciatic nerve was transected in 15 Sprague-Dawley rats. A 15-mm Silastic tube (Dow Corning, Midland, MI) was placed between the ends of the cut nerve and filled with either laminin, aFGF, or buffer applied to collagen sponges. RESULTS: Ten weeks postimplantation, mean axon counts showed that both laminin (2432) and aFGF (1612) produced significantly higher numbers of axons than controls (1009) (P < .05) and that laminin showed significantly more nerve regeneration than aFGF (P < .05). CONCLUSION: These results indicate that laminin and aFGF enhance peripheral nerve regeneration across a large gap, presumably through their neurotrophic effects and mitogenic properties on supporting cells. Furthermore, it is concluded that the transient nature of aFGF's effect on the regenerative environment limits its effectiveness at regenerating axons over a prolonged period of time.
Authors: Manning J Sabatier; Bao Ngoc To; Samuel Rose; Jennifer Nicolini; Arthur W English Journal: J Neurophysiol Date: 2011-11-02 Impact factor: 2.714
Authors: David J Bryan; C Robert Litchfield; Jeffrey V Manchio; Tanya Logvinenko; Antonia H Holway; John Austin; Ian C Summerhayes; Kimberly M Rieger-Christ Journal: Proteome Sci Date: 2012-02-10 Impact factor: 2.480
Authors: Cyril Pottier; Ligia Mateiu; Matthew C Baker; Mariely DeJesus-Hernandez; Cristina Teixeira Vicente; NiCole A Finch; Shulan Tian; Marka van Blitterswijk; Melissa E Murray; Yingxue Ren; Leonard Petrucelli; Björn Oskarsson; Joanna M Biernacka; Neill R Graff-Radford; Bradley F Boeve; Ronald C Petersen; Keith A Josephs; Yan W Asmann; Dennis W Dickson; Rosa Rademakers Journal: Brain Date: 2022-07-29 Impact factor: 15.255